Method development and validation of paracetamol drug by RP-HPLC
Journal Title: Journal of Medical and Allied Sciences - Year 2013, Vol 3, Issue 1
Abstract
A simple and reproducible method was developed for paracetamol by Reverse Phase High Performance Liquid Chromatography (RP-HPLC). Paracetamol was separated on C18 column [4.6x250mm, particle size 5μm], using ortho phosphoric acid buffer with pH of 3.5 at the UV detection of 207nm. Isocratic elution of acetonitrile (ACN) and water was used as a mobile phase with various ratios and flow rates, eventually 25:75 v/v ACN and water was being set with the flow rate of 1mL/min. The statistical validation parameters such as linearity, accuracy, precision, inter-day and intra-day variation were checked, further the limit of detection and limit of quantification of paracetamol concentrations were found to be 120ng/mL and 360ng/mL. Recovery and assay studies of paracetamol were within 99 to 102% indicating that the proposed method can be adoptable for quality control analysis of paracetamol.
Authors and Affiliations
T. A. Phazna Devi| Department of Genetics & Biotechnology, Osmania University, Hyderabad-500 007, Andhra Pradesh, India, Aravind Setti| Department of Genetics & Biotechnology, Osmania University, Hyderabad-500 007, Andhra Pradesh, India, S. Srikanth| Department of Genetics & Biotechnology, Osmania University, Hyderabad-500 007, Andhra Pradesh, India, Sivaramaiah Nallapeta| Onan Centre for Molecular Investigations, Miryalaguda, Secunderabad-500 047, Andhra Pradesh, India, Smita C. Pawar| Department of Genetics & Biotechnology, Osmania University, Hyderabad-500 007, Andhra Pradesh, India, J. Venkateshwara Rao| Department of Chemistry and Zoology, Nizam College, Osmania University, Hyderabad-500 004, Andhra Pradesh, India
Keywords
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