Method Development and Validation of RP-HPLC Method for Simultaneous Estimation of Olmesartan Medoxomil and Hydrochlorothiazide in Bulk and Pharmaceutical Dosage Form
Journal Title: International Journal of Pharma Research and Health Sciences - Year 2014, Vol 2, Issue 6
Abstract
Present work describes a simple, rapid, precise, accurate, economical and reproducible reverse phase high performance liquid chromatographic (RPHPLC) method and its validation for the estimation of Olmesartan medoxomil and Hydrochlorothiazide in bulk and pharmaceutical dosage form on a Develosil ODS HG-5 RP C18, (5m, 15cmx4.6mm i.d.) using a mixture of Potassium dihydrogen phosphate buffer+Dipotassium hydrogen phosphate (0.02 M, pH 5.0): acetonitrile (40:60) %v/v as mobile phase in an isocratic elution mode, at a flow rate of 1.0 ml/min. The detection was monitored at 258 nm.The retention times of Olmesartan and Hydrochlorothiazide were found to be 9.93 min and 2.02 min respectively.Excellent linearity range was found between 0-60 μg/ml for Olmesartan and Hydrochlorothiazide. Proposed method was validated with respect to linearity, precision, accuracy, specificity and ruggedness. Method was successfully applied for the simultaneous determination of Olmesartan and Hydrochlorothiazide from the combined dosage form.
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