Method Development and Validation on Etomidate injection by RP-HPLC
Journal Title: International Journal of Pharmacy and Analytical Research - Year 2012, Vol 1, Issue 1
Abstract
A new simple, accurate, rapid and precise isocratic High performance liquid chromatographic (HPLC) method was developed and validated for the determination of Etomidate (ETO) injection. The Method employs Waters HPLC system on Develosil –ods-UG column (300 x 3.9 mm x 5µm) and flow rate of 1.5 mL/min with a load of 20 µL. Acetonitrile and Phosphate buffer was used as mobile phase in the composition of 40:60. The Detection was carried out at 254 nm. Linearity ranges for Etomidate was 40-240 µg/ml respectively. Retention Time of Etomidate was found to be 12.061 minutes respectively. Percent recovery study values of Etomidate were found to be within 98-102 %. This newly developed method was successfully utilized for the Quantitative estimation of Etomidate in injectables. This method was validated for accuracy, precision, linearity and Robustness as per ICH guidelines.
Authors and Affiliations
Kalpana Gopagani
Development and Validation of Uv-Spectrophotometric Method for Estimation of Abacavir Sulfate Nanoparticles.
A simple, precise and accurate spectrophotometric method has been developed for the determination of Abacavir Sulfate nanoparticles. Standard Stock solution was prepared in pH 7.4 phosphate buffer and further dilution we...
Analytical method Development and Validation for the estimation of Pioglitazone hydrochloride in Bulk and Tablet dosage form by UV_Spectroscopy
This paper describes the analytical method suitable for validation of Pioglitazone hydrochloride by UV Spectrophotometric method. The method utilized UV spectroscopy and the solvent system was consists of 6 N Glacial ace...
In-vivo analgesic, anti-inflammatory and central nervous system - locomotor activities of certain synthesized 2-[(1-((phenyl amino) methyl) substituted 1H-benzoimidazol-2-yl) alkyl] isoindoline-1, 3-diones
Two homologous series of 1, 2-disubstituted benzimidazoles carrying isoindolines (6a-n) were synthesized by mannich type reaction of 2-alkyl benzimidazolyl isoindoline-1,3-dione (4a-b) with different substituted aromatic...
Formulation and Evaluation of Microspheres of Cefdinir
Microspheres are sub-micron size polymeric drug carrier systems in which the therapeutic agents are loaded in micrometer. These particles consist of core material, which is the drug, and a coating material. Microspheres...
Consumer’s Perception Regarding Pharmaceutical Product Packaging: A Survey of Pakistan
The packaging of the pharmaceutical product plays an important role to maintain the product stability and an increase in sales which ultimately increase in profit margin of any organization. The packaging continuously pr...