Method development and validation using HPLC technique – A review
Journal Title: Journal of Drug Discovery and Therapeutics - Year 2014, Vol 2, Issue 10
Abstract
Analytic method development and validation are key elements of any pharmaceutical development program. HPLC analysis method is developed to identify, quantity or purifying compounds of interest. This article mainly focuses on the optimization of HPLC conditions and other important perspectives during method development and validation. Various critical steps related to analytical method development and validation is discussed. A sequence of events required for method development and analytical validation are described. The steps involved in developing a stability-indicating HPLC method influences the analysis of degradation products/impurities in stability study and its validation demonstrate the suitability for its intended purpose.
Authors and Affiliations
Suman Sood| Department of Pharmaceutics, Rayat Institute of Pharmacy, Rail Majra S.B.S Nagar, Punjab, India, Rajni Bala| Department of Pharmaceutics, Rayat Institute of Pharmacy, Rail Majra S.B.S Nagar, Punjab, India, N. S. Gill| Department of Pharmacology, Rayat Institute of Pharmacy, Rail Majra S.B.S Nagar, Punjab, India
Psoriasis Cure with Homoeopathy
Psoriasis is a chronic autoimmune condition that causes the rapid build-up of skin cells. This build-up of cells causes scaling on the skin’s surface. Inflammation and redness around the scales is fairly common. Typical...
A REVIEW ON ADHESIONS AND THEIR PREVENTION
Adhesion formation is a physiological consequence of peritoneal tissue repair. Surgical trauma of the peritoneal surfaces induces a sequence of events that effectuates wound healing, therefore can also ultimately leads t...
SMILE ON YOUR FACE DURING WIKI INTER-FACE
The website www.wikipedia.org is now becoming crazy among the web hunting persons of modern era. The new horizon of wired generation is proceeding towards with the running Encyclopædias by the frequent usage of webglossa...
Possible Study of Drug-Drug Interactions between Lisinopril and Gliclazide in Experimental Animals
The most often problem in type II diabetic patients coexists with hypertension, the incidence of hypertension among diabetic patients is high. In this case, use a combination of medicine unavoidable (Polypharmacy). In su...
Wound healing activity of the ethanolic extract of Solanum spirale leaves indigenous to north east India
As a part of present work objective are to investigate indigenous plant used in wound healing in northeast India, we hereby reported our findings related to wound healing activities of plant Solanum spirale (Solanaceae)...