Method development and validation using HPLC technique – A review
Journal Title: Journal of Drug Discovery and Therapeutics - Year 2014, Vol 2, Issue 10
Abstract
Analytic method development and validation are key elements of any pharmaceutical development program. HPLC analysis method is developed to identify, quantity or purifying compounds of interest. This article mainly focuses on the optimization of HPLC conditions and other important perspectives during method development and validation. Various critical steps related to analytical method development and validation is discussed. A sequence of events required for method development and analytical validation are described. The steps involved in developing a stability-indicating HPLC method influences the analysis of degradation products/impurities in stability study and its validation demonstrate the suitability for its intended purpose.
Authors and Affiliations
Suman Sood| Department of Pharmaceutics, Rayat Institute of Pharmacy, Rail Majra S.B.S Nagar, Punjab, India, Rajni Bala| Department of Pharmaceutics, Rayat Institute of Pharmacy, Rail Majra S.B.S Nagar, Punjab, India, N. S. Gill| Department of Pharmacology, Rayat Institute of Pharmacy, Rail Majra S.B.S Nagar, Punjab, India
Formulation and Evaluation of Nanosilver Loaded Silk Fibroin Topical Gel
The skin is providing barrier to the outside protecting the body from infection, radiation, and extremes of temperature. There are various types of wounds which can damage the skin including abrasions, lacerations, ruptu...
A review on self emulsifying drug delivery system: promising approach to enhance bioavailability
Among the approaches to improve the oral bioavailability of the molecules, the use of self-emulsifying drug delivery system has been shown to be reasonably successful in improving the oral bioavailability of poorly water...
"PROSPECTIVE ANALYSIS OF PREVALENCE AND EFFECT OF LIVER DISEASES IN TYPE II DIABETES PATIENTS: A CROSS SECTIONAL, OPEN LABEL, SINGLE CENTRIC, PILOT STUDY”
That Patients Who has a Liver disease, May because of type 2 diabetes .In cirrhosis Patients diabetes may be occur. In diabetic patients cirrhosis may be occur diabetic play an important role of liver disease. In Diabeti...
JAHN-TELLER DISTORTIONS: A NEW STRATEGY IN QUANTUM MECHANICS
The concepts of the Jahn-Teller effect and vibronic coupling are being applied to more and more systems in both chemistry and physics. Aspects of structural chemistry such as the distortion of the nuclear framework to a...
Dendrimer as a carrier for ocular drug delivery
The foremost challenges face by today’s researcher is ocular drug delivery. Delivery of drug to the targeted ocular tissue is restricted by various ocular barriers. To conquer the ocular drug delivery barriers and get be...