METHOD DEVELOPMENT FOR SIMULTANEOUS ANALYSIS OF MARKER SCOPOLETINE, ANDROGRAPHOLIDE, QUERCETIN, AND LUTEOLIN IN ANTIHYPERTENSION JAMU FORMULATION USING RP-HPLC
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 3
Abstract
Objective: The aim of this research was to develop a method of simultaneous analysis marker compounds in antihypertension jamu formulation using reverse phase high performance liquid chromatography (RP-HPLC).Methods: Stationary phase, mobile phase, sample preparation and instrument condition were optimized.Results: Analysis of marker compounds in anti hypertension jamu formulation using HPLC with reverse phase ODS C18 column (LiChroCART®, 125 x 4.6 mm, particle size of 5 µm) and gradient mobile phase of formic acid 1% and methanol could separate markers in 35 min at flow rate 2 mL/min. Four markers in antihypertension jamu formulation have been successfully analyzed simultaneously, were scopoletine, andrographolide, quercetin, and luteolin. The RSD values demonstrated the intra and inter-day precisions were less than 4% and 6.5% respectively and the recoveries around 93.45-114.42%, respectively. All calibration curves showed good linear correlation coefficient which of R > 0.995.Conclusion: RP-HPLC method has been developed for simultaneous analysis of four markers in antihypertension jamu. The methods can be used to analyze antihypertension jamu formulation in the market.Â
Authors and Affiliations
Ummi Rukoyah, Irda Fidrianny, Komar Ruslan W
Keywords
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