Method for Constructing Human iPS Cell Line RZPBi001 from Peripheral Blood Cells
Journal Title: Journal of Clinical & Cellular Immunology - Year 2023, Vol 14, Issue 3
Abstract
Patient-derived hiPSCs could generate indefinitely illness-related cells and organoids, allowing research scientist to reproduce some pathologic change of human sickness models in petri dishes. Whereas, dermal fibroblasts require some traumatic injury to obtain and have the risky of accumulating variations due to unveil to external stimulus such as UV light. Meanwhile, peripheral blood is regarded to be a more excellent source of somatopiasm because it is less damage to collect and its mutation load is significantly lower than that of skin tissue. In this paper, we describe the successful construction of non-integration hiPSCs form human peripheral blood cells by Sendai virus. A healthy adult female of Han nationality from China contributed her Peripheral Blood Mononuclear Cells (PBMCs). Non-integrating Sendai virus recombinant PBMCs with human OSKM (Oct4, Sox2, Kl4 and c-Myc) transcription factors. The pluripotency of transgenic-free iPSCs was confirmed by immunofluorescence staining of pluripotency markers and the capacity of iPSCs to initially differentiate into 3 germ layers in vitro. Moreover, iPSC lines demonstrated awfully normal karyotypes. In the research of illness progression and pathogenesis (eg, DMD, SMA, ALS), iPSC lines can be used as controls.
Authors and Affiliations
Xiaoqing Chen Yuling Lua Rong Ying Weiming Yua Pengfei Tian Dongming Hua Yu Feng
Keywords
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- EP ID EP741781
- DOI 10.35248/2155-9899.23.14.686.
- Views 29
- Downloads 0
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