METHOD FOR DETERMINATION OF AMINO ACID CONTENT IN PROTEIN PRODUCTS FOR MEDICAL USE
Journal Title: Revista de Pielărie Încălţăminte - Year 2017, Vol 17, Issue 4
Abstract
The paper presents a method for determination of amino acid content in collagen biomaterials for medical use produced in the Collagen Department of INCDTP - Division ICPI and its validation. The method has three stages: the first consists in hydrolysing the collagen biomaterial sample down to amino acids; the second step refers to derivatization of amino acids with N, O-bis (trimethylsilyl) trifluoroacetamide (BSTFA) which is a silylation reagent; derivatized amino acids are detected using a mass spectrometer after gas chromatography. The method was validated to establish performance parameters and to check compliance with the intended purpose.
Authors and Affiliations
Gabriela MACOVESCU, Ciprian CHELARU, Dana GURĂU
Keywords
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- EP ID EP533219
- DOI 10.24264/lfj.17.4.8
- Views 112
- Downloads 0
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