Methodical approaches to the radial hemolysis reaction use for equine infectious anemia diagnostics
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2018, Vol 32, Issue
Abstract
Introduction. Of Equine Infectious Anemia (EIA) is one of the most important issue that constantly registers in many countries with the developed horse breeding. Diagnostics of EІA is a basic element of the preventive measures system. The basic serum method of diagnostics is a reaction of diffuse precipitation (Coggins test). In accessible sources, we did not find any reports on the radial hemolysis reaction in EIA diagnostics, as well as the necessary guidelines on the technique of its performance. The goal of the work. To conduct a study on the possibilities to use the method of radial hemolysis in the diagnosis of EIA. To establish optimal conditions for its implementation and influence of various factors on its sensitivity. Materials and methods. The EIA virus strain "З-ВІЕВ-К" was cultivated on initial and subculture of horse embryos organs and passaged culture of trachea of calf (ТC). Immune sera against EIA virus were used from the test-kit «A set for the diagnosis of infectious anemia of horses in the reaction of diffuse precipitation (RDP)» produced by FSE «Shchelkovo Biocombinat» (RF). RDP was performed in the following sequence: erythrocytes sensibilization complement preparation, preparation of test wells, samples introduction into the test wells, account of the results. Results of research and discussion. It is established that the method has advantages over the conventional RDP-test in the EIA diagnosis, namely: the possibility of using low-concentration viral preparations and high specificity. Conclusions and prospects for further research. For the improvement of epizootic situation on EIA it is necessary to conduct the continuos diagnostic researches with the maximal scope of horse population and performe preventive measures to control this disease.
Authors and Affiliations
Paul Kryvoshya, Oleg Rud
Keywords
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- EP ID EP405704
- DOI 10.31073/vet_biotech32(2)-37
- Views 108
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