Methylation Status of SOX17 and RUNX3 Genes in Acute Leukemia
Journal Title: Iranian Journal of Blood and Cancer - Year 2015, Vol 7, Issue 5
Abstract
Background: Several studies have examined the presence of DNA methylation of CpG islands in leukemia. Methylation of SOX17 and RUNX3 genes may play a role in leukemogenesis through silencing tumor suppressor genes. We investigated the methylation status of SOX17 and RUNX3 genes in patients with acute leukemia. Methods: In this case-control study, peripheral blood samples from 100 AML and 100 ALL patients and 100 healthy controls were collected. Isolated DNA was treated with sodium bisulfite and methylation status was examined by methylation specific PCR (MS-PCR) with primers specific for methylated and unmethylated sequences of SOX17 and RUNX3 genes. Results: The frequency of hypermethylation of SOX17 and RUNX3 genes were 36% and 28%I in patients with acute myeloid leukemia (AML), and 21% and 22% in patients with acute lymphoblastic leukemia (ALL), respectively. Aberrant methylation of these genes was found in all FAB classifications of AML and ALL. Hypermethylation of SOX17 (P=0.055) and RUNX3 (P=0.003) genes were associated with FAB-M0 and M1 subtypes of AML, respectively. Also, aberrant methylation of RUNX3 gene was associated with FAB-L1 subtype of ALL (P=0.053). There was not any significant association between hypermethylation of SOX17 and RUNX3 genes and clinical parameters of patients with leukemia including sex, age, WBC, and platelet counts. Conclusion: Hypermethylation of SOX17 and RUNX3 genes was seen in patients with acute leukemia. Moreover, no significant association was observed between hypermethylation of SOX17 and RUNX3 and induction of remission.
Authors and Affiliations
Ali Ghasemi, Abbas Ghotaslou, Kazem Ghaffari, Mohsen Mohammadi
Keywords
Related Articles
- EP ID EP281465
- DOI -
- Views 59
- Downloads 0
Non-pharmacological Interventions for Needle-related Procedural Anxiety in Children with Thalassemia
Background: The purpose of the present study was to assess the efficacy of non-pharmacological interventions on the anxiety among thalassemic children undergoing venipuncture. Patients and Methods: This was a clinical tr...
Molecular Markers in Neuroblastoma
Neuroblastoma, one of the common malignant childhood tumors, arises from neuroblast cells derived from the neural crest and destined for the adrenal medulla and the sympathetic nervous system and shows remar...
Making plasma derived medicines available
Recent progress in past two decades in recombinant technology has created the opportunity of production of some of the plasma proteins including co -agulation factors using mammalian cell cultures. However, some limitati...
Results of the Pathology of Spleen in 1492 Patients Undergoing Splenectomy
Spleen is a major component of the reticuloendothelial system and functions as a filtering system for the encapsulated bacteria especially in children. Knowing the spectrum of conditions leading to splenectomy in any geo...
Patterns of Gelatinase-B Expression in Leukemic Cell Lines
Background: Gelatinase-B named MMP-9 (matrix metalloproteinase-9), is a protease that degrades collagen type ІV and V of extracellular matrix. MMP-9 production is increased in various types of cancers including leukemia...