Microarray analysis of altered gene expression in diallyl trisulfide-treated HepG2 cells.
Journal Title: Pharmacological Reports - Year 2005, Vol 57, Issue 6
Abstract
Diallyl trisulfide (DT) is a natural compound derived from garlic. Despite its reported lipid-lowering effects, the mechanisms of its actions are not clear yet. To further understand the molecular mechanisms of actions of this compound, microarray technology was used in the present study to investigate the lipid-lowering effects of DT on HepG2 cells. To optimize the concentration of DT treatment on HepG2 cells, a series of concentration of DT were incubated with HepG2 cells for 24 h. The data indicted that the concentrations of DT in the range from 20 to 50 muM were effective in lowering cellular total triglyceride and cholesterol, with no significant cytotoxicity. Using oligonucleotide microarrays and RT-PCR technology, the genes that were differentially regulated by DT were identified. The results showed that peroxisome proliferator activated receptor alpha (PPAR-alpha) and hepatocyte nuclear factor 4alpha (HNF-4alpha) mRNA were up-regulated, and CYP7A1 mRNA was down-regulated following DT treatment, suggesting that the lipid-lowering effects of DT may be at least in part mediated through the regulation of PPAR-alpha dependent pathways.
Authors and Affiliations
Zhe Zhou, Hong-Ling Tan, Bing-Xin Xu, Zeng-Chun Ma, Yue Gao, Sheng-Qi Wang
Keywords
Related Articles
- EP ID EP159767
- DOI -
- Views 110
- Downloads 0
Involvement of prolactin and somatostatin in depression and the mechanism of action of antidepressant drugs.
Neuropeptides have been implicated in the physiology and pathophysiology of stress responses and therefore may play an important role in the pathogenesis of affective disorders such as Major Depression Disorder (MDD). Th...
Mechanisms of [Ca(2+)]i elevation following P2X receptor activation in the guinea-pig small mesenteric artery myocytes.
Background: There is growing evidence suggesting involvement of L-type voltage-gated Ca(2+) channels (VGCCs) in purinergic signaling mechanisms. However, detailed interplay between VGCCs and P2X receptors in intracellula...
Effect of caffeine on the anticonvulsant effects of oxcarbazepine, lamotrigine and tiagabine in a mouse model of generalized tonic-clonic seizures.
Caffeine has been reported to be proconvulsant and to reduce the anticonvulsant efficacy of a variety of antiepileptic drugs (carbamazepine, phenobarbital, phenytoin, valproate and topiramate) in animal models of epileps...
Search for drugs of the combined anti-inflammatory and anti-bacterial properties: 1-methyl-N'-(hydroxymethyl)nicotinamide.
It has already been reported that 1-methylnicotinamide (MNA(+)), a primary metabolite of nicotinamide (vitamin B(3)), possesses remarkable anti-inflammatory properties [3]. This communication shows that 1-methyl-N'-(hydr...
Kinetic studies of the effects of Temodal and quercetin on astrocytoma cells.
The aim of the present study was to investigate the kinetics of the effects exerted by Temodal and quercetin on the survival of the human astrocytoma MOGGCCM cell line. Our results indicate that quercetin was toxic and i...