Microbial Oil Derived from Filamentous Cyanobacterium Trichormus sp. as Feedstock to Yield Fatty Acid Ethyl Esters by Enzymatic Synthesis
Journal Title: Journal of Advances in Biology & Biotechnology - Year 2017, Vol 12, Issue 4
Abstract
The aim of this study was to optimize the cultivation conditions of Trichormus sp. CENA77 and to evaluate the lipid feedstock to generate ethyl esters via enzymatic route using NovozymÒ 435 as catalyst. Under optimized cultivation conditions (1.5 g L-1 Na2CO3 and 150 µmol photon m−2s−1 light intensity), biomass productivity of 286.5 ± 3.6 mg.L−1.day−1, lipid contents of 14.5 ± 2.8% and lipid productivity of 41.5 ± 0.4 mg.L−1day−1 were achieved. Enzymatic transesterification was performed in a microwave reactor using a 1:12 molar ratio (oil/ethanol) at 50°C assessing iso-octane as solvent for 12 h. The viscosity values for the microbial oil (51.9 mm2.s-1) sharply decreased to 10.7 mm2s-1, upon the progress of transesterification reaction. The maximum fatty acid ethyl esters yield (80%) achieved was due to the presence of non-lipid compounds, which may have affected the biocatalyst activity. Evidence of the Novozym® 435 deactivation was demonstrated by the assistance of Fourier transform infrared spectroscopy and scanning electron microscopy analyses.
Authors and Affiliations
Patrícia C. M. Da Rós, Caroline S. Pamplona Silva, Maria Estela Silva-Stenico, Marli F. Fiore, Heizir F. de Castro
Keywords
Related Articles
- EP ID EP318106
- DOI 10.9734/JABB/2017/33045
- Views 75
- Downloads 0
Biotechnology as a Cradle of Scientific Development: A Review on Historical Perspective
Historically, the science of biotechnology started through domestication of plants and animals by early men and selective plant breeding which subsequently led to transgenesis. Fermentation of grains and fruits using yea...
Genetic Diversity of Soybean Yield Based on Cluster and Principal Component Analyses
The objective of this study was to determine analysis of variance, descriptive statistics, cluster and principle components analysis to understand their genetic diversity for ten soybean genotypes on seed yield (ten/fed....
Preparation of Different Refused Derived Fuel Combinations with Domestc Sewage Sludge to Enhance Gross Calorific Value
The objective of the present study is to maximize the resource value. The need to minimize disposal has prompted efforts within sewage sludge management to utilize sewage sludge as a commodity. The present study describe...
Investigation of Triticale and Wheat Performance under Dry Land Conditions on the Basis of Variations in Agronomic and Morphological Traits
Aims: Drought is the most important environmental stresses in arid and semi-arid regions worldwide. Triticale offers ample genetic variation for the improvement of its productivity under limited water conditions. In orde...
Implication of Testosterone in Human Puberty Attainment
This study evaluated and compared the serum levels of testosterone in male and female subjects aged 6—10 years. It also assessed the number of children among the subjects who could be potential candidates for precocious...