Microbial Production of Silver Nanoparticles (Agnps) By Some Bacterial Isolates
Journal Title: IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB - Year 2018, Vol 4, Issue 4
Abstract
The five bacterial strains viz. Brevibacillus centrosporus DSM8445T, Brevibacillus invocatus NCIMB13772T, Brevibacillus choshinensis DSM8552T, Brevibacillus panacihumi DCY35 and Brevibacillus levickii LKG22481were screened for production of AgNPs using their extracellular (cell free supernatant) of bacterial cultures. The primary sight for AgNPs formation was change of colour of reaction mixture from pale yellow to dark brown. In the present investigation it is evident that isolated bacterial strain Brevibacillus invocatus NCIMB13772T was the most efficient bacterial strain in production of AgNPs (0.996) followed by isolated bacterial strain Brevibacillus centrosporus DSM8445T (0.960), Brevibacillus panacihumi DCY35 (0.920), Brevibacillus levickii LMG22481 (0.382) and Brevibacillus chosinensis DSM8552T (Table- 2). The primary conformation of synthesis of nanoparticles in the medium was characterized by the changes in color from yellowish white to brown , the knowledge about the reduction of silver ions and formation of silver nanoparticles were still not clear, but it is believed that protein molecules and enzyme, the nitrate reductase act as good regulating agent in silver nanoparticles synthesis. The biosynthesis of AgNPs by these five bacterial strains has also been confirmed by FTIR, XRD and SEM techniques.
Authors and Affiliations
Amar Kumar, Baidyanath Kumar, Ashok Ghosh, Mini Tiwari, Muddassir Reyaz
Keywords
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- EP ID EP415140
- DOI 10.9790/264X-0404022638.
- Views 125
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