Microbial removal of engine oil from polluted media (the case study: Hamadan City)
Journal Title: Journal of Advances in Environmental Health Research (JAEHR) - Year 2017, Vol 5, Issue 2
Abstract
Discharging industrial wastes into the soil causes accumulation of pollutants, especially petroleum hydrocarbons and used engine oil, in the environment. This study was done to find some bacterial strains capable of removing the engine oil from contaminated soils. Soil samples contaminated by engine oil were collected from some polluted area of Hamadan City, and then their bacterial strains were isolated and classified on the basis of morphological keys and biochemical tests. The efficiency of these bacterial strains in removing the pollutant was evaluated in minimal salt medium (MSM) containing 1% engine oil. The optical density (OD) of the media was measured as an indicator of bacterial growth and degradation of the engine oil during day 0, 5, 10, and 15 at 600 nm wavelength. The bacterial strain responsible for the highest OD was chosen as the effective one, and the efficiency of oil removing was evaluated for this bacterial strain. In this study, eight bacterial strains called EODB1-8 were isolated. The results of the OD determination showed that the four bacterial strains caused more turbidity in the samples. The effects of time, type of bacteria, and their interaction effects were also significant in all samples. The turbidity of the samples was increased with increasing exposure time. The maximum turbidity was caused by the bacterial strain EODB4 and it was introduced as the best engine oil degrading bacterial strain. Its engine oil removal efficiency was evaluated to be 62.85% and it was identified as Pseudomonas alcaligenes using morphological keys and biochemical identification methods.
Authors and Affiliations
Fariba Mohsenzadeh, Sanaz Safaie
Keywords
Related Articles
- EP ID EP425290
- DOI 10.22102/jaehr.2017.80690.1011
- Views 89
- Downloads 0
Metal bioaccumulation, oxidative stress, and biochemical alterations in the freshwater snail (Galba truncatula) exposed to municipal sewage
Urban wastewater contains various detergents, pesticides, pharmaceutical medications, cosmetics, hygiene products, and heavy metals. Discharge of municipal sewage into surface water affects the health of aquatic organism...
The simultaneous removal of turbidity and humic substances from water using the enhanced coagulation process
This study aimed to investigate the efficiency of the enhanced coagulation (EC) process for the simultaneous removal of turbidity and humic substances (HS) from raw water from the Sanandaj Water Treatment Plant (SWTP). T...
Photocatalytic efficiency of molybdenum-doped zinc oxide nanoparticles in treating landfill leachate
Among various techniques available for leachate treatment, nano-photocatalytic-based techniques have been considered as efficient. The photocatalytic leachate treatment using nanoparticles of zinc oxide doped with molybd...
Synthesis and structural properties of Mn-doped ZnO/Graphene nanocomposite
Zinc oxide (ZnO) is a promising metal oxide semiconductor with various applications, especially in the photocatalytic destruction of environmental pollutants. However, this nanoparticle has some limitations, such as poor...
Hydrothermal synthesis of surface-modified copper oxide-doped zinc oxide nanoparticles for degradation of acid black 1: Modeling and optimization by response surface methodology
Dyes are widely used in various industries most of them are not readily biodegradable and are consisted of number of toxic, mutagenic, and carcinogenic compounds. Therefore, it is essential to remove them from effluent b...