Microbicidal Activity of Neutrophils Isolated from HIV Patients
Journal Title: International Blood Research & Reviews - Year 2015, Vol 3, Issue 3
Abstract
HIV infection is associated with a progressive loss of T cell functional capacity and reduced responsiveness to antigenic stimuli. Neutrophils are crucial cellular components of the innate immune system. Current study evaluates the functional activity of neutrophils isolated from HIV/AIDS patients with similar clinical laboratory parameters differing only in the use of antiretroviral therapy (HAART). Two patients HIV/AIDS patients, a female and a male, were selected for this study, based on clinical history, general physical examination and laboratory tests. Further, two apparently healthy volunteers of the same age and gender former de control group. Neutrophils isolated from human peripheral blood were placed in contact with the yeast in a proportion of 1:10 leukocytes for 1 hour. PMN Fluorescence was detected in FL1 on a flow cytometer and results were recorded as fluorescence intensity and percentage of positive cells in the sample. HOCl formation was monitored by spectrophotometry based on the resulting taurine chloramine-forming reaction of hypochlorous acid with taurine. The experiments revealed that patient I with HAART had a 17.3% lower response activity of neutrophils when compared with the control in the production of hypochlorous acid with PMA stimulation. Patient II, who did not use HAART, was 81% less active than the control in the production of hypochlorous acid. The two patients had similar clinical laboratory parameters differing only in the number of CD4 cells, which was higher in Patient II. Results show that the patient submitted to antiretroviral treatment had a better quality of functional response of neutrophils although with fewer CD4 cells.
Authors and Affiliations
A. R. T. Pupulin, J. B. Borges, T. Sakurada Jr, N. C. S. Santana, S. Lautenschlaugher
Keywords
Related Articles
- EP ID EP242453
- DOI 10.9734/IBRR/2015/16929
- Views 121
- Downloads 0
Gliricidia sepium Aqueous Leaf Extract Possesses Antisickling Property
Background: Extracts of leaf, flower and bark of Gliricidia sepium have been used in the treatment of patients with pathogenic bacterial infections, skin diseases, nematodes and antioxidants disturbances. We recently had...
Comparative Effect of Chilli Pepper (Capsicum frutescens) Extract and Capsaicin on Some Haematological Parameters and Serum Electrolytes in Albino Wistar Rats
Aim: Capsaicin is the active ingredient in chilli pepper, and is responsible for the pungency of chilli pepper. This study compared the effect of ethanolic extract of chilli pepper fruit and capsaicin on haematological p...
Level of Pentraxin-3 in Patients with Acute Leukemia in Septicemia and Its Prognostic Value
Introduction: In acute leukemia, sepsis is potentially fatal. Pentraxin3 is a protein rapidly produced in response to primary inflammatory signals. It shows high levels in sepsis, specially associated with vascular and e...
Prevalence of Anaemia among Children Attending Paediatrics Department of UDUTH, Sokoto, North-Western Nigeria
Introduction: Anaemia is a global public health problem. It affects more than 56 million people globally, two thirds of them being from developing countries. This study was designed to investigate the prevalence and type...
A Probe into Protective Association of Haemoglobin Genotypes with Malaria Parasitaemia among Students of a University in Western Delta, Nigeria
Aim: This work was designed to investigate any possible protective association of haemoglobin genotypes with malaria parasitaemia among students of a private University in Western Delta. Study Design: Whole blood sample...