Micronuclear Assay in Petrol Pump Workers: A Prospective Observational Study
Journal Title: Scholars Academic Journal of Biosciences - Year 2017, Vol 5, Issue 8
Abstract
The presence of micronuclei in exfoliated buccal epithelial cells has proven a useful biomarker of occupational exposure to petroleum products. Genetic damage is the most important basic cause of developmental and degenerative disease. It is also well established that genomic damage is produced by environmental exposure to genotoxins, medical procedures (eg, radiation and chemicals), micronutrient deficiency (eg, folate), lifestyle factors (eg, alcohol, smoking, drugs, and stress), and genetic factors, such as inherited defects in DNA metabolism and/or repair. To investigate the cytogenetic damage in exfoliated buccal cells obtained from petrol pump attendants and control subjects, using the micronucleus test. The study sample consisted of 100 subjects (males) in the age group 21-60 years (Exposed group). The control group consisted of 100 individuals without clinically observed lesions and without any tobacco habits (Unexposed group). The petrol pump attendants (Exposed) group consisted of 50 subjects with tobacco habits such as tobacco chewing only, smoking only and both tobacco chewing and smoking(Exposed Group I); other 50 exposed subjects included were petrol pump workers without the above-said habits (Exposed Group II). The smear was obtained from the mucosa of the oral cavity by using the sterile cytopathology brush. Slides fixed in ethanol fixative and stained using Giemsa and PAP stain and 1000 cells were studied by using a light microscope. In this study, it was found that an average number of micronuclei (MN) were directly proportional to the years of exposure, 08.77 in subjects with 14 years of exposure and least in less exposed subjects (3-5 years). The average number of micronuclei (MN) in subjects who worked for more than 12 hours a day was high (08.23). A higher frequency of micronuclei was observed in petrol pump workers when exposed for a longer duration and much higher MN were seen in workers with tobacco use. It is necessary to educate the petrol pump workers about the hazardous and genotoxic effects so as to ensure the safety and healthy working atmosphere.
Authors and Affiliations
Dr. Sridhar Reddy Erugula, Dr. Badari Ramakrishna, Dr. Jesudass Govada, Dr. K. Prudhvi Krishna, Dr. Mohammed Aziz ur Rahman, Dr. Shahid Imran, Dr. Ayesha Sameera, Dr. Deepika Veldurthy
Keywords
Related Articles
- EP ID EP398199
- DOI -
- Views 77
- Downloads 0
Association between Serum Homocysteine Concentration in Pregnant Women and Neonatal Birth Weight
Abstract: Maternal total homocysteine concentrations have been linked to a wide range of adverse pregnancy outcomes and could possibly influence birth weight. Keeping this in mind the present study was done to find the a...
Extensive Intraductal Component (EIC) Positive Carcinoma of Breast-Four Years’ Experience at a Cancer Centre
Abstract:Extensive intraductal component positive carcinoma (EICPC) of breast is a well defined entity. Nevertheless few data exist concerning their characteristics and prognostic behaviour. Our objective was to describ...
Oral Lichenoid Lesion and Amalgam Restoration
Lichen planus (LP) is a chronic autoimmune disease of the skin and or mucous membranes and when the etiology is known and it involves the oral mucosa it is called oral lichen lesion. Most common site is the buccal mucosa...
In vivo effect of pimpinella anisum seeds essential oils on Mouse mice BAL b/c breed infected with Listeriosis disease
Pimpinella anisum L. (Apiaceae) is an annual herb indigenous to the near east and widely cultivated in the Mediterranean regions as well as Mexico and Chile. It has been used as an aromatic herb and spice since Egyptian...
The Versatile Silicone: Case Report
Management of patients with facial deformities require multidisciplinary approach. Maxillofacial prosthetic materials are used to replace facial parts lost through disease, trauma or congenital deformity. Silicone rubber...