Micronucleus assay in formalin exposed individuals
Journal Title: Indian Journal of Clinical Anatomy and Physiology - Year 2018, Vol 5, Issue 1
Abstract
Introduction The aim of the study was to find out the nuclear abnormalities like Micronuclei in the oral mucosa of the formalin exposed individuals in the department of anatomy This MN Aaasy is an inexpensive methodMaterials and Methods Faculties and staffs working in the department of anatomy and the firstyear students were included in this study Study sample consists of 50 subjects and was divided into 5 groups according the years of exposure By scraping with the wooden spatula in the buccal mucosa Oral squamous cells were collected and smeared on the slides The smeared slides were fixed with methanol glutaraldehyde fixative and stained with giemsa maygrunwald stain After airdrying 1000 cells were screened for Micronucleus MNResults Micronuclei frequency was more in the group who had more than 15 years of formalin exposure The mean MN count was 960 and it was statistically significant when compared to other groupsConclusion Micronucleus Assay is the most simple feasible inexpensive method to find out the nuclear abnormalities at an early stage in the formalin exposed individualsKeywords Formalin Buccal Mucosa Micronucleus
Authors and Affiliations
N. Pratheepa Sivasankari, S. Sundarapandian, E Sakthivel
Keywords
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- EP ID EP475354
- DOI 10.18231/2394-2126.2018.0019
- Views 50
- Downloads 0
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