Micronucleus Investigation in Buccal Mucosal Cells of Young Waterpipe Tobacco Smokers in Tehran
Journal Title: Journal of Pharmaceutical & Health Sciences - Year 2017, Vol 5, Issue 3
Abstract
Waterpipe Tobacco Smoke(WTS) is an unhealthy life style that may increase the risk of genotoxic responses and chronic diseases such as cancer. Micronucleus test is a successful and reliable method which is used for screening of genetotoxic responses of whole body and also for screening those people who had already exposed to genotoxic compounds. In this study, specific questionnaires were designed and used for studying the role of shisha smoking on the extent of genotoxic responses and cases were looked for MNs with this biomonitoring method. The study population was 20 young adults (12men and 8 women) who born and lived in Tehran and had continuously smoked shisha more than 2 times weekly for more than 2 years . The associations between all recorded background, environmental and nutritional factors and increased incidence of Micronucleus in buccal cells of all cases were considered by statistical methods. In order to count Micronucleus levels, buccal cells were collected from buccal mucosa of these people with small-headed toothbrush and was placed the head of tooth brush into buccal cell buffer, slides were prepared and cells were stained with Schiff’s reagent and light green .Finally 1000 differentiated cells were recorded by optical microscope in each slide and the mean level of MN was determined for each volunteer . All steps were performed according to the buccal micronucleus cytome (BMCyt) assay protocol. Increased incidence of Micronucleus was associated with the extent of shisha smoking per week (p=0.021), alcohol consumption ( p=0.021) and BMI ( p=0.027). The other effective factor in the occurrence of Micronucleus was gender/sex ( p=0.011) but nutritional factors didn’t change the level of Micronucleus in our cases. The relationship between other background and environmental factors were not significant too. It seems that long term consumption of shisha in both genders could increase the risk of genetic toxicity and occurrence of malignancies in human target cells.
Authors and Affiliations
Sepideh Arbabi Bidgoli
Keywords
Related Articles
- EP ID EP242929
- DOI -
- Views 129
- Downloads 0
Production of Clavulanic Acid by Streptomyces clavuligerus in Batch Cultures with Using Wheat Bran as the Source of Carbon
Nowadays Microbial biotechnology is considered as one of the most important and profitable branches of industry. In this approach, by using the generator microorganisms, We are able to produce biotechnological products,...
Comparison the effect of carboxymethyle cellulose films containing Thymus vulgaris and Zataria multiflura on shelf life of chicken meat
Poultry meat is a kind of perishable food. The growth of pathogenic microorganisms may occur in chicken meat during storage in the refrigerator. Microbial growth causes a serious hazard to the safety of the food consumer...
Preparation of ZnO and ZnS nanoparticles and in-vitro study of their antimicrobial effect
Zinc sulfide (ZnS) & zinc oxide (ZnO) nanoparticles was evaluated for their antimicrobial activity against four pathogenic strains. ZnS&ZnO nanoparticles were synthesized by simple aqueous chemical reaction in an aqueous...
Physicochemical characteristics and storage stability of clarified butter fat « smen » produced from pasteurized and non-pasteurized milk
Smen is a milk fat isolated by ‘boiling-off’ water. Milk fat was first converted into butter and then into smen. Smen product is utilized for culinary cooking and frying of different food. Smen has better shelf-life than...
Physicochemical Analysis of Margarine, Butter and Butter Oil Samples of Iran’s Markets
Butter, margarine and butter oil are three products which consume daily by Iranian community. Detection of type and existence of synthetic antioxidants (SAs) quantities in the margarine, butter, and butter...