Micropropagation and Conservation of Fig (Ficus Carica L.)

Apply

Micropropagation and Conservation of Fig (Ficus Carica L.)

Journal Title: JOURNAL OF ADVANCES IN AGRICULTURE - Year 2019, Vol 10, Issue 0

Abstract

An efficient protocol is outlined for rapid and mass micropropagation of Ficus carica L. (fig). Shoot tips (5 mm) were obtained from mother plants stock grown on half strength Murashige and Skoog (½ MS) medium with the addition of 30 g/L sucrose. For shoot multiplication Benzyl amino purine (BAP) and kinetin produced differences number of new shoot per plant and shoot height. BAP at 0.4 mg/L in combination with 0.2 mg/L indole-3-butyric-acid (IBA) produce maximum in vitro propagation rate, with 4.2 shoots per ex-plant. Root initiation was experimented on MS medium containing different concentrations of mg/L, IBA, IAA (Indole-3-acetic-acid) (IAA) or Naphthalene acetic acid (NAA). Highest number of root (4.3) was resulted when 1.5 mg/L IBA was used. After acclimatization in a mixture of (1 soil: 1 perlite: 1 peat) survival rate of 80% was achieved. For in vitro conservation of F. carica was experimented as microshoots were stored for 40 weeks on MS medium containing different sucrose concentration. Medium supplemented with 3% sucrose gave the highest regrowth (89%) at 24 ± 2 °C. Culture grew slowly when transferred to new fresh medium after the storage periods.

Authors and Affiliations

Mohamad Shatnawi, Rida A. Shibli, Wesam G. Shahrour, Tamara S. Al-Qudah, Abu-Zahra Taleb

Keywords

COST OF MILK PRODUCTION AND BREAK EVEN ANALYSIS OF MEMBER AND NON MEMBER OF DAIRY COOPERATIVE SOCIETY FOR MILCH ANIMALS (COW & BUFFALO) IN DISTRICT ETAWAH OF U.P.

This study covered 150 cooperative member milk producers and 150 non-member milk producers which were post- stratified into Landless, Marginal, small, medium and large herd size categories. Breakeven point is a point whe...

EP ID EP652473
DOI 10.24297/jaa.v10i0.8160
Views 139
Downloads 0