MICROPROPAGATION OF Centella asiatica THROUGH TISSUE CULTURE AN ATTEMPT TO DEVELOP AN EFFICIENT PROTOCOL FOR LARGE SCALE PRODUCTION
Journal Title: Indian Journal of Scientific Research - Year 2018, Vol 8, Issue 2
Abstract
Centella asiatica of Apiaceae is an important medicinal herb, found in the moist shady places as a wild species. Because of its medicinal value people do harvest the species from its natural population. This plant has been listed for immediate conservation by the Medicinal Plant Board, Govt. of India. Keeping this idea in mind tissue culture study was done to select suitable combination and concentration of the growth regulators so that it may be considered as an efficient protocol that shall be exploited at commercial scale to provide large number of uniform planting material. MS basal medium was supplemented with 3% sucrose, seven different concentrations of BAP and KN with 0.5 mg/l NAA, separately. It was noted that MS + 3% sucrose, gelled with 0.8% agar + 4.0 mg/l BAP + 0.5 mg/l NAA gave the highest percentage of response that was 92.64% with respect to shoot bud initiation which was 4.68 in number after 12th day of inoculation. At the similar concentrations KN + NAA gave only 58.84% response, with 2.24 number of shoot buds after 16th day of inoculation. On subculture in the same culture conditions MS + 4.0 mg/l BAP + 0.5 mg/l NAA induced 8.72 numbers of axillary shoots per explants. 4 week old plantlets were used for rooting in MS + 0.3 to 1.5 mg/l IBA. Here again the maximum number of roots was obtained in MS + 0.5 mg/l IBA which was 4.5 / plantlet on an average.
Authors and Affiliations
RITIKA KUMARI
Keywords
Related Articles
- EP ID EP477463
- DOI -
- Views 81
- Downloads 0
STUDY OF LUMBAR LORDOSIS AND PELVIC POSITION IN BHARATANATYAM DANCERS
Bharatanatyam is one of the most ancient dance forms in India. This dance style is characterized by a linear form of the body without any pronounced movement of the upper body and linear spatial patterns, which make the...
APEXIFICATION WITH RAPID MTA PLUG TECHNIQUE
Immature permanent teeth create an endodontic challenge for complete asepsis and three dimensional obturation of the root canal system. Apexification forms an apical barrier or a stop against which one can place root can...
MONITORING OF BACTERIOLOGICAL QUALITY OF DRINKING AND TAP WATER FROM A TERTIARY CARE HOSPITAL IN EASTERN INDIA USING A NEW MEDIUM
Bacteria pathogenic for gut, present in drinking water, pose a great risk to laboratory workers. They should be monitored and reported promptly . It is generally measured by growing the water in MacConkey broth. We here...
PATALAGANGA PROJECT -AGAINST HYDROGEOLOGICAL CHARACTERISTICS OF KARNATAKA, INDIA
The proposal of ‘Water Quest Hydroresources Management Limited (WQH)’ to tap water through deep bore wells from concept based resources located deep in the earth crust as ‘Water Veins/Underground Rivers’ was for its cons...
A theoretical investigation of corrosion of mild steel in acidic medium by triazole based Schiffs base as inhibitor
The inhibitive performance of three triazoles namely diamino triazole (TA), anisaldine 3, 5-diaminotriazole (ATA), vanilidine 3,-diaminiotriazole (VTA) have been studied using DFT approach. The inhibitorsinvestigated in...