MICROPROPAGATION OF Centella asiatica THROUGH TISSUE CULTURE AN ATTEMPT TO DEVELOP AN EFFICIENT PROTOCOL FOR LARGE SCALE PRODUCTION

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MICROPROPAGATION OF Centella asiatica THROUGH TISSUE CULTURE AN ATTEMPT TO DEVELOP AN EFFICIENT PROTOCOL FOR LARGE SCALE PRODUCTION

Journal Title: Indian Journal of Scientific Research - Year 2018, Vol 8, Issue 2

Abstract

Centella asiatica of Apiaceae is an important medicinal herb, found in the moist shady places as a wild species. Because of its medicinal value people do harvest the species from its natural population. This plant has been listed for immediate conservation by the Medicinal Plant Board, Govt. of India. Keeping this idea in mind tissue culture study was done to select suitable combination and concentration of the growth regulators so that it may be considered as an efficient protocol that shall be exploited at commercial scale to provide large number of uniform planting material. MS basal medium was supplemented with 3% sucrose, seven different concentrations of BAP and KN with 0.5 mg/l NAA, separately. It was noted that MS + 3% sucrose, gelled with 0.8% agar + 4.0 mg/l BAP + 0.5 mg/l NAA gave the highest percentage of response that was 92.64% with respect to shoot bud initiation which was 4.68 in number after 12th day of inoculation. At the similar concentrations KN + NAA gave only 58.84% response, with 2.24 number of shoot buds after 16th day of inoculation. On subculture in the same culture conditions MS + 4.0 mg/l BAP + 0.5 mg/l NAA induced 8.72 numbers of axillary shoots per explants. 4 week old plantlets were used for rooting in MS + 0.3 to 1.5 mg/l IBA. Here again the maximum number of roots was obtained in MS + 0.5 mg/l IBA which was 4.5 / plantlet on an average.

Authors and Affiliations

RITIKA KUMARI

Keywords

Centella asiatica Apiaceae Micropropagation Tissue Culture Growth Regulators Axillary Shoots

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