Micropropagation of Cerasus avium (L.) Moench in vitro
Journal Title: Лісівництво і агролісомеліорація - Year 2014, Vol 124, Issue
Abstract
The paper deals with peculiarities of micropropagation of cherry (Cerasus avium (L.) Moench). The potential for initiating and proliferating of shoot cultures derived from C. avium and a micropropagation method for these species was developed. The high efficiency of the method for the foundation stock sterilization for introduction into culture in vitro developed by Laboratory of Forest Tree Breeding of URIFFM earlier was identified. Shoot apices and axillary buds were collected in laboratories from greenhouse-grown one-year-old trees of cherry. Nodal segments, 0,5-1 cm in length, without leaves were then excised from the shoots. The effects of different plant growth regulators in micropropagation and medium that encourages axillary bud development were investigated. In order to identify the influence of apical dominance on the intensity of formed shoots surveys separately for the terminal and axillary buds are being conducted. Morphogenesis activity for explants of green nodal segments of shoots and apical meristem was high (60.8 to 100 %) regardless of the type of phytohormones. In proliferation stage, multiplication rate of about 1,4 was achieved over a 8 week period using MS medium with 0,3 mg l -6-Benzylaminopurine.
Authors and Affiliations
N. Vysotska
Keywords
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