Micropropagation of Helichrysum italicum (ROTH) G. Don – a medicinal plant with ornamental value
Journal Title: Journal of BioScience and Biotechnology - Year 2018, Vol 7, Issue 2
Abstract
Helichrysum italicum (Roth) G. Don is a Mediterranean vegetal species from the Asteraceae family. The ornamental value of the flowers and the properties of its essential oils contribute to its popularity. The aim of the present study was to compare the effect of different cytokinins on the multiplication of Helichrysum italicum and to develop a reliable protocol for in vitro micropropagation of this medicinal and ornamental plant. Nutrient media based on both MS (Murashige and Skoog, 1962) or DKW (Driver and Kuniyuki, 1984) formulations were used. They were enriched with different cytokinins - BAP, Kinetin, or Zeatin (5μM). The best multiplication rate was achieved on DKW medium, supplemented with 5μM Kinetin. A high percentage of rooting was achieved on the control treatment and the nutrient media, supplemented with IBA.
Authors and Affiliations
Nataliya Dimitrova, Lilyana Nacheva
Keywords
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