Abstract

Nodal explants from forced cuttings of Patharnakh were used for micropropagation protocol development. The effect of different media {1/2 MS (M 1 ), MS (M 2 ) and WPM (M 3 )} and growth regulators (BAP, IBA and NAA) on establishment, proliferation and rooting was examined. Necrotic culture (%) was found to be influenced by type of media and growth regulator fortification during establishment stage. Lowest necrotic cultures (0.00%) were observed in M 2 medium fortified with BAP (1.0 mgl -1 ) and IBA (0.01 mgl -1 ). Maximum establishment (96.29%) was obtained on M 2 medium fortified with BAP (1.5 mgl -1 ) and IBA (0.01 mgl -1 ). Maximum proliferated cultures (85.67%) were obtained using M3 medium fortified with BAP (2.5 mgl -1 ). Similarly the highest shoots per explant (3.80) were obtained in M 3 medium supplemented with BAP (2.5 mgl -1 ). Shoots of maximum length (53.75) were obtained in M 3 medium containing BAP (0.0 mgl -1 ), i.e., control. Rooting (%) was maximum (10.16%) using M 1 medium fortified with IBA (1.0 mgl -1 ). No rooting was obtained irrespective of media using NAA. Roots per explant were 2.38 using M 1 medium supplemented with IBA (1.0 mgl -1 ). However, roots of maximum length were obtained using M 3 medium supplemented with IBA (1.0 mgl -1 ).

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