Micropropagation of Phalaenopsis amabilis cv. Cool 'Breeze' with using of flower stalk nodes and leaves of sterile obtained from node cultures
Journal Title: International Journal of Farming and Allied Sciences - Year 2014, Vol 3, Issue 7
Abstract
In this study we tried to produce this flower by micropropagation method with use of flower stalk nodes and leaves of sterile which are obtained from node cultures as explant. We examined different experiments such as sterilization of nodes with different concentration of sodium hypochlorite for introduction of the best method for sterilization and the influences of different concentration of BA and NAA on MS media for shoot formation, IAA and NAA on 1:2 MS media for root induction and NAA, BA and TDZ for the production of seedlings on the Jen medium. The results shown that the best assembly for sterilization is about 7% sodium hypochlorite. The highest shoot formation achieved 15.3, produced planet from one node, obtained from MS medium contained 4.40 mgL-1 BA and 1 mgL-1 NAA. The best result earned from root induction about 2.45 roots, each planet from 1:2 MS media contained 1 mgL-1 IAA. Protocorms were gradually emerged from cultivated leaf sides at sixth week of the culture. Results indicated that with augmented concentration on TDZ the production of protocorms increased, but enlarged concentration on TDZ had profound consequence in reducing the active sample. The lowest losses of protocorm belonged to the supplement of 0.5mg/l TDZ (0.66) and the highest deprivation achieved from treatment of TDZ complement with 3mg/l. The best adoption (93%) effectively performed in the second medium (cocopit, coal, industrial cartridge + the bites of yonolit in volume ratio 1+1+2+4), In the first medium (cocopit + coal in volume ratie 5 to 1) and also 90.2% of explants were survived.
Authors and Affiliations
Khosro Balilashaki ,Ruhangiz Naderi, Siamak Kalantari and Aboozar Soorni
Keywords
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