Micropropagation of the Monopodial Orchid, Rhynchostylis retusa (L.)
Journal Title: International Journal of Life Science - Year 2018, Vol 6, Issue 1
Abstract
Rhynchostylis retusa, a monopodial orchid is important for both its medicinal and economic values. Different parts like shoot, leaf and root were used as explants for micropropagation of Rhynchostylis retusa. The explants were cultured in Vacin and Went (VW), Knudson C and Nitsch media supplemented with sucrose (20 g L-1) and growth regulators, viz., NAA (0.1 mgL-1), BAP (1.0 mgL-1) and Kinetin, Kn (0.4-1.0 mgL-1) in different concentrations and combinations. Green protocorm-like bodies (PLBs) and calli which later differentiated into plantlets were induced from shoot-tip, leaf and root-tip explants using VW medium supplemented with NAA (0.1 mgL-1) along with either Kinetin (1.0 mgL-1) or BAP (1.0 mgL-1). Maximum survival as well as differentiation was obtained from shoot-tip explants. Further growth and development of PLBs into plantlets were achieved when cultured in VW medium with gradual removal of NAA and reduction of Kinetin and BAP concentrations. Transplantation in community pots could be done when the plantlets were 3.0-3.5 cm. These results suggest a methodology for in vitro mass propagation of Rhynchostylis retusa.
Authors and Affiliations
Sunitibala Devi Y, Neelashree N
Keywords
Related Articles
- EP ID EP545346
- DOI -
- Views 96
- Downloads 0
Assessment of disease intensity and isolates characterization of blast disease (Pyricularia oryzae CAV.) from South West of Ethiopia
Blast (Pyricularia oryzae Cav.) disease is one of the most important biotic constraints to rice production in South West of Ethiopia. The present study was conducted to determine the intensity of the blast disease and th...
Faunal Diversity of CSIR-NEERI campus, Nagpur, Maharashtra, India
CSIR-NEERI is a constituent of council of scientific and industrial research and is situated in the center of Nagpur city. CSIR-NEERI is located at coordinate 2107’21’’ N and 7904’17’’ E and NEERI colony located at coord...
Incidence of fungal spores around garbage depot of Mulund with reference to health hazards
Air pollution is due to the presence of particulate matters e.g. varying mixtures of soot, ash, pollen grains, fungal spores, microscopic plant & animal fragments, dust etc. Aerobiologists have traditionally been involve...
Significance and Management of Chickpea Wilt/root rot and Future Prospects in Ethiopia. A Review
Chickpea production and productivity in Ethiopia has recently declined and high potential yield gaps because of several abiotic and biotic factors. The average chickpea yield in Ethiopia is usually below 2 t/ha although...
An exploration of species composition of vertebrate fauna of Orchha Wildlife Sanctuary, Central India
This research is an exploration of vertebrate fauna of the Orchha Wildlife Sanctuary (OWS) in central India. The study consolidates information from field surveys. A total of 15 fish (6 families), 6 amphibians (3 familie...