Microsatellite DNA marker in aquatic organisms
Journal Title: Scientific Journal of Biological Sciences - Year 2013, Vol 2, Issue 9
Abstract
For the last 50 years, attempts have been made to discriminate amongpopulations by using molecular markers. Although some techniques have provedsuccessful in certain circumstances, the consistent trend to newer markersamong fishery geneticists highlights the general lack of resolving powerobserved with older technologies. The last three decade has seen the increasinguse of satellite DNA in investigations of genetic variability and divergence. Thelarge interest in microsatellite loci is largely due to the very high levels ofvariability that have been observed and the ability to investigate thisvariation using PCR technology. The isolation and application ofmicrosatellites to research fields as diverse as population genetics, parentageanalyses and genome mapping are reviewed. Statistical considerations (e.g.appropriate sample sizes, number of loci and the mutation model assumptions onwhich the estimate is based) have not been considered in detail yet and theproblems are often exacerbated in aquatic species, as some species show verylarge numbers of alleles at microsatellite loci
Authors and Affiliations
A. Moradi*| Department of fishery, Marine Sciences and Technology of Khoramshahr University, Iran., S. Keyvanshokooh| Department of fishery, Marine Sciences and Technology of Khoramshahr University, Iran.
Keywords
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- EP ID EP1091
- DOI 10.14196/sjbs.v2i9.1001
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