Microsporogenesis manipulation by CHA’s to eliminate emasculation in hybrid seed production of okra (Albemoschus esculemtum L.)
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2017, Vol 5, Issue 0
Abstract
Present study was conducted to identify effective microsporogeneis and flower bud stage for application of male gametocides to induce pollen sterility in okra. Results of study revealed the association of floral bud size and stages of microsporogenesis in female parental lines. Formation of microspore from PMC is between 3 mm to 5 mm bud size. Further, it was reported that Meiosis I completed when but is under 5 mm and after this microgametogenesis start appearing when the bud size between 10 mm to 40 mm buds. However, pollen remains nonviable till bud reaches 45mm size and attains maturity just before flower anthesis between 45mm to 50 mm bud size. Days taken in formation of microspore to viable pollen grain are 18-20 days. Male gametocides used in the study were effective in inducing pollen sterility and vary with different stages of microsporogeneis and bud growth. Among the different treatment combinations, 45 mm bud size treated with malic hydrazide at 450 ppm induced higher pollen sterility under in vitro pollen germination test (93.40 %) and this was followed by the acetocarmine (90.77 %) and ethrel @ 3000 ppm (80.68) at 45 mm bud size.
Authors and Affiliations
Naveen N, Pallavi HM, Satish D, Sarvamangala S Cholin, VM Ganiger, Babu AG
Keywords
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- EP ID EP428131
- DOI 10.18006/2017.5(5).623.630
- Views 57
- Downloads 0
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