MIMOSA PUDICA EXERTS NEUROPROTECTION AGAINST MPP+INDUCED NEUROTOXICITY IN SHSY5Y CELL LINES-AN IN VITRO MODEL OF ANTI-PARKINSONISM
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 9, Issue 1
Abstract
Objective: Parkinson’s disease (PD) is one of the of a neurodegenerative disorder, It’s decreased the dopaminergic neurones, tyrosine hydroxylase (TH) and increased the α-synuclein protein level. This study was conducted to investigate the neuroprotective effect of Mimosa pudica have the abilities to improve TH and DAT proteins expression against MPP+ induced neurotoxicity, in in vitro model of Parkinson’s disease using SH-SY5Y human neuroblastoma cell lines. Methods: Mimosa pudica were pre-treated with various concentration for cell viability assay. Vehicle alone or Mimosa pudica (300µg) for 24 h, and then were co-treated with 1000µM MPP+ for 15 min in the continued presence of vehicle or Mimosa pudica. After treatment, cells were collected for protein expression.Results: Cell viability assay confers the inhibitory concentration cell death of Mimosa pudica. MPP+significantly down-regulated the protein expression of TH (p<0.01) and DAT (p<0.05). Mimosa pudica decreased the expression of α synuclein (p<0.01) in MPP+ intoxicated cell lines.Conclusion: The present study showed that Mimosa pudica exerts neuroprotection by suppressing α synuclein and the dopaminergic neurdegeneration. Mimosa pudica may be due to quecertin which might be acted via the anti-oxidant mechanism. The above finding suggests that Mimosa pudica may act as a potential target in the management of PD. Â
Authors and Affiliations
Mahadevan M. V. , Ramaswamy R. S. , Banumathi V.
Keywords
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- EP ID EP575743
- DOI 10.22159/ijpps.2017v9i1.15728
- Views 90
- Downloads 0
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