MINIMUM INHIBITORY CONCENTRATION OF SOME 6,7,8,9-TETRAHYDRO-5H-[1,2,4]TRIAZOLO[4,3-A]AZEPINE DERIVATIVES AGAINST AMMONIFYING BACTERIA ISOLATED FROM THE SOIL FERROSPHERE
Journal Title: Biota. Human. Technology - Year 2023, Vol 1, Issue 1
Abstract
Soil ammonifying bacteria play an important role both in creating conditions for the course of the microbial corrosion process and in the metal corrosion process itself. Compounds of 6,7,8,9-tetrahydro-5H-[1,2,4]triazolo[4,3-a]azepine, with quaternary nitrogen exhibit high antibacterial properties by the agar diffusion method. The method of serial dilution of compounds in a liquid nutrient medium allows to estimate the minimum antibacterial concentration of compounds.The aimof the study was to study the sensitivity of ammonifying bacteria isolated from the soil ferrosphere to some derivatives of 6,7,8,9-tetrahydro-5H-[1,2,4]triazolo[4,3-a]azepine using the dilution method.The study was carried out by the methodof serial dilutions generally accepted in microbiology using a 4-day associative culture of ammonifying bacteria previously isolated from the microbial community of the ferrosphere of a corroding steel pipe. The predominant representatives of the association were Bacillus simplex ChNPU F1, Fictibacillus sp. ChNPU ZVB1, Streptomyces gardneri ChNPU F3, Streptomyces canus NUChC F2. The culture medium was meat-peptone broth; the cultivation temperature was 29 ± 2 ºС. The studied compounds − bromide 3-anilinomethyl-1-[2-(4-chlorophenyl)-2-oxoethyl]-6,7,8,9-tetrahydro-5H-[1,2,4]triazolo[4,5-a]azepinium, bromide 1-[2-(4-chlorophenyl)-2-oxoethyl]-3-(4-tolylaminomethyl)-6,7,8,9-tetrahydro-5H-[1,2,4]triazolo[4,3-a]azepinium, bromide 1-(2-oxo-2-phenylethyl)-3-(4-tolui-dinomethyl)-6,7,8,9-tetrahydro-5H-[1,2,4]triazolo[4,5-a]azepinium.Scientific novelty-the sensitivity of ammonifying bacteria isolated from the soil ferrosphere to some derivatives of 6,7,8,9-tetrahydro-5H-[1,2,4]triazolo[4,3-a]azepine was investigated using the dilution method, which clarifies the antibacterial properties of the compounds, namely their minimum inhibitory concentration.Conclusions-the culture of ammonifying bacteria is sensitive to the studied quaternary triazoloazepine salts, which in concentrations of 10.3-123.5 μg/mL showed bacteriostatic properties. The highest sensitivity was noted for compound II (bromide 1-[2-(4-chlorophenyl)-2-oxoethyl]-3-(4-tolylaminomethyl)-6,7,8,9-tetrahydro-5H-[1,2,4]triazolo[4,3-a]azepinium, the minimum inhibitory concentration of which is 10.3 μg/mL. A further perspective is the determination of the minimum bactericidal concentration of quaternary triazoloazepine salts, in particular, in relation to other ecological and physiological groups of bacteria -agents of microbiologically influenced corrosion.
Authors and Affiliations
Nataliia Tkachuk, Viktor Yanchenko, Nataliya Demchenko
Keywords
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- EP ID EP753459
- DOI https://doi.org/10.58407/bht.1.23.2
- Views 21
- Downloads 1
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