MiR-33a and statins collaboratively reduce the proliferative capacity of prostate cancer cells
Journal Title: The European Research Journal - Year 2018, Vol 4, Issue 4
Abstract
Objectives: Prostate cancer (PCa) is one of the leading causes of cancer deaths among men in the developed countries. Accumulating data suggests a high-cholesterol Western diet as an important risk factor for PCa. Besides,significant evidencesassociate increased serum cholesterol levels with PCa development and progression.In this study, we aimed at investigating the collaborative roles of cholesterol analogs, cholesterol-lowering drugs, and miR-33a, which is an important microRNA involved in regulation of cholesterol metabolism,on the cellular phenotypes associated with PCa progression. Methods: We evaluated the effects of low-density lipoprotein (LDL) cholesterol, 25-hydroxycholesterol (25-HC), mevastatin and simvastatin on their ownand together with miR-33a on the proliferation, invasion and anchorage independent growthcapacity of PCa cells using Cell Counting Kit-8, Matrigel invasion, and soft agar assays, respectively. Results: We show that cholesterol analogs significantly promoted proliferative, invasive, and clonogenic potential of PCa cells, while cholesterol loweringstatins demonstrated opposite effects. Moreover, LDL and 25-HC reversed the tumor suppressive potential of miR-33a and statin treatment promoted the proliferation inhibitory effect of miR-33a on PCa cells. Conclusions: We demonstrated that statins inhibited the cellular phenotypes associated with PCa progression and miR-33a treatment strengthens the impacts of statins on cellular proliferation. These findings suggest that statins alone and together with miR-33a might be a useful tool for effective and successful eradication of PCa cells.
Authors and Affiliations
Ömer Faruk Karataş, Michael Ittmann
Keywords
Related Articles
- EP ID EP392657
- DOI 10.18621/eurj.380619
- Views 113
- Downloads 0
Correlation of serum C-reactive protein and procalcitonin levels in infections of kidney transplant recipients
Objectives. Procalcitonin is a propeptide of calcitonin and has been increasingly used as a biomarker of infection. The aim of this study was to evaluate correlation of serum C-reactive protein (CRP) and procalcitonin (P...
Pathological analysis of cystic lesions of the bones: a retrospective single-center 10-year overview
Objective: Many different neoplastic and non-neoplastic lesions involve the skeletal system. Clinical and radiological tools primarily assess the nature of these lesions.The aim of this study was to analyze the cystic bo...
The frequency of nodular gastritis in adult patients and the relation of this type of gastritis with Helicobacter pylori and histopathologic findings
Objectives: Endoscopic nodular gastritis is usually used to describe the miliary nodular appearance of the gastric mucosa during endoscopy. Recent studies have shown that nodular gastritis is closely related to Helicobac...
Class I pentalogy of Cantrell: a rare case report
Pentalogy of Cantrell is characterized by the presence of five major congenital defects involving the abdominal wall, pericardium, diaphragm, lower sternum and various cardiac abnormalities. This rare syndrome was descri...
Could there be any relationship between nutritional deficiencies and idiopathic chest pain in children?
Objectives: Chest pain in childhood is a frequent cause of referral to pediatric cardiology departments although cardiac etiology is very rare. Etiology is usually unidentified and named as ‘idiopatic chest pain’. It is...