Mitochondrial Oxygen Consumption by the Foreskin and its Fibroblast-rich Culture
Journal Title: Sultan Qaboos University Medical Journal - Year 2013, Vol 13, Issue 3
Abstract
Objectives: Tis study investigated the feasibility of using a phosphorescence oxygen analyser to measure cellular respiration (mitochondrial O2 consumption) in foreskin samples and their fbroblast-rich cultures. Methods: Foreskin specimens from normal infants were collected immediately after circumcision and processed for measuring cellular respiration and for culture. Cellular mitochondrial O2 consumption was determined as a function of time from the phosphorescence decay of the Pd (II) meso-tetra-(4-sulfonatophenyl)-tetrabenzoporphyrin. Results: In sealed vials containing a foreskin specimen and glucose, O2 concentration decreased linearly with time, confrming the zero-order kinetics of O2 consumption by cytochrome oxidase. Cyanide inhibited O2 consumption, confrming that the oxidation occurred mainly in the mitochondrial respiratory chain. Te rate of foreskin respiration (mean ± SD) was 0.074 ± 0.02 μM O2 min-1 mg-1 (n = 23). Te corresponding rate for fbroblast-rich cultures was 9.84 ± 2.43 μM O2 min-1 per 107 cells (n = 15). Fibroblast respiration was signifcantly lower in a male infant with dihydrolipoamide dehydrogenase gene mutations, but normalised with the addition of thiamine or carnitine. Conclusion: Te foreskin and its fbroblast-rich culture are suitable for assessment of cellular respiration. However, the clinical utility of foreskin specimens to detect disorders of impaired cellular bioenergetics requires further investigation.
Authors and Affiliations
Fatma Al-Jasmi| Department of Paediatrics, College of Medicine & Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates, Tachillath Pramathan| Department of Paediatrics, College of Medicine & Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates, Adnan Swid| Tawam Hospital, Al Ain, United Arab Emirates, Bahjat Sahari| Tawam Hospital, Al Ain, United Arab Emirates, Harvey S. Penefsky| Faculty of Medicine & Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates, Abdul-Kader Souid| Department of Paediatrics, College of Medicine & Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates
Keywords
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