MODERN METHOD OF PROPAGANATION OF CHICORY SALAD
Journal Title: Овочівництво і баштанництво - Year 2019, Vol 1, Issue 65
Abstract
The aim of the research. The article outlines materials for studying the peculiarities of propaganation of chicory salad edivy and escariols in vitro. Methods. The study of the peculiarities of propaganation of chicory of salad edivy and eskariols in vitro was conducted in the lab microclonal reproduction of the National Dendropark "Sofiivka" of the NAS. In the conducted researches it was envisaged to select the source plant material (seed) for introduction of in vitro; sterilization of the plant material and introduced into the culture of in vitro; picked up nutritional media with the addition of growth regulators; hemogenesis and seedlings of secondary explants; rhizogenesis of explants; adaptation of regenerators to ex vitro conditions. Results. The conducted researches have established, that method of microclonal propaganation in vitro was one of the perspective parts of technology of cultivation of chicory salad edivy and eskariols. As a result of studies, it was found that the most effective (85.2%) was 1-minute sterilization of explants with mercuric dichloride solution (HgCl2). For the use of silver nitrate sterility was 63.4%, and for treatment with sodium mercury -58.2%. Increasing the exposure of sterilization reduced the yield of sterile seeds due to damage to the seed bud. The best indicators - 65.4 - 72.8% were obtained for the propagation of explants in medium MS 3 with a concentration of 0.5 mg/L BAP and 0.1 mg/L NAA. For induction of rhizogenesis, the MS 2 nutrient medium with a concentration of 0.5 mg/l IBA was found to be most effective. The reproduction coefficient for the studied varieties of chicory salad was 12.1 - 20.4. They proved that there were no significant differences in the hemogenesis in chicory varieties of salad edible and escario. It was established that the largest number of rooted microcloons (88.7%) was obtained using modified medium of MS 2 with addition at a concentration of 0.5 mg/l IBA, where the number of formed roots was 8.1 pp. Increasing the concentration to 1.0 mg/l led to a decrease in rhizogenesis. For complex use in the medium of IAA and NAA in different concentrations, there was a decrease in the number of rooted explants. Conclusions. Studies of the adaptation conditions of the rooted regeneration plants found that the effective methods were the adaptation of the test plants using the Eco-plus substrate universal. Resiliency of plants-regenerators was 81.8 – 88.9 %.
Authors and Affiliations
O. I. Ulianych, S. V. Shchetina, G. Y. Slobodyanyk, O. D. Lukyanets, L. I. Voivoda, O. V. Kukhnyuk
Keywords
Related Articles
- EP ID EP643022
- DOI 10.32717/0131-0062-2019-65-58-65
- Views 41
- Downloads 0
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