MODIFICATION OF NUTRICULTURE MEDIUM FOR INDUCING CALLUSOGENESIS IN VITRO OF CAMELINA SATIVA
Journal Title: Наукові доповіді НУБіП України - Year 2018, Vol 1, Issue 1
Abstract
Camelina sativa is a promising agricultural crop of multi-use. Introduction of new high-quality varieties is important to increase the volume of crop production. The use of biotechnological methods makes it possible to reduce significantly the duration of selection process and increase the efficiency of creating new plant forms with desirable economic and valuable features. One of the first stages of in vitro research is to obtain callus biomass in sufficient quantities with high morphogenic parameters as the main source material for the creation of new genotypes. A number of factors influence the process of callusogenesis. The purpose of our research is to determine the optimal composition of nutriculture medium and its modifications to induce callusogenesis in vitro of camelina sativa. The work was carried out in the biotechnological laboratory of Uman National University of Horticulture. Segments of camelina sativa seedlings of Stepovyi 1 variety were used as explants. Murashige and Skoog, Schenck and Hildebrant and Gamborg nutriculture media were modified with growth regulators of auxin (2.4-dichlorophenoxyacetic acid) and cytokinin (6-benzylaminopurine) at concentrations of 0.1; 0.5; 1.0; 1.5 mg/l. The effectiveness of each variant of the nutriculture medium was evaluated by the activity of proliferation and morphogenic characteristics of callus tissues. There is no initiation of callus tissues on the non hormonal media. The most intense callusogenesis of camelina sativa was carried out for introduction of 0.1 mg/l 2.4-D and 1.0 mg/l 6-BAP to the nutritional substrate. In Murashige and Skoog nutriculture medium, the proportion of explants with dedifferentiation and proliferation of callus tissues was 76.6 %. In Schenck and Hildebrant and Gamborg nutriculture media, the indicator was 27–45 % lower. Calluses were average filled with water, white or light green, having loose or semi-solid consistency with a large number of morphogenically active cells. The increase in the concentration of 2.4-dichlorophenoxyacetic acid caused the inhibition of explant tissues of camelina sativa, significantly reduced the intensity of callusogenesis and morphogenetic characteristics of calluses. On the basis of the dispersion analysis, it is found that 2.4-D concentration (65.8 %) and the ratio of growth regulators in the nutriculture substrate (17.9 %) had the greatest influence on the induction of callusogenesis. The share of influence of the nutriculture medium was only 5.6 %. Consequently, during the research, the nutriculture medium was modified to obtain the callus tissue of camelina sativa with high morphogenic characteristics.
Authors and Affiliations
I. O. Lybchenko, A. I. Lybchenko, L. O. Ryabovol
Keywords
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