Modification of Solubility Test for the Diagnosis of Sickle Cell Disease
Journal Title: Journal of Medical Science And clinical Research - Year 2017, Vol 5, Issue 1
Abstract
Mass screening for sickle cell disease is very much needed. A screening test should be cheap, easy to perform, less cumbersome and should have high sensitivity, low specificity is accepted. TOT (turn over time, meaning time taken for a single cycle of test, this excludes time taken for sample collection) should be short. Solubility test is good enough but it is not regularly done for its low sensitivity and specificity. Sickling test needs trained microscopist/ technician. TOT is also long, up to 24 hrs. Agarose gel electrophoresis is an art, needs good technician, costly, instrumentation, consumables, TOT is long, and cannot differentiate between HB S,C,D disease in alkalinepH . Cellulose acetate electrophoresis is similar to agaroe gel but much more costlier than agarose gel. HPLC is a costly affair not suitable in indian scenario. With some modifications, (Modified ) Solubility Test corrects the fallacies of normal solubility test
Authors and Affiliations
Dr Satyabrata Patra
Keywords
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