Modified Ninhydrin reagent for the detection of amino acids on TLC plates
Journal Title: Journal of Applied and Natural Science - Year 2013, Vol 5, Issue 1
Abstract
Ninhydrin is the most well known spray reagent for identification of amino acids due to its high sensitivity. But, it produces same purple/violet color with all amino acids, except proline and hydroxy proline. A new spray reagent, para bromobenzoic acid has been introduced here which produces distinguishable colors with the amino acids with moderately high sensitivity (0.01-1.0) µg. A probable mechanism for such color formation has also been proposed
Authors and Affiliations
Amalendu Sinhababu
Keywords
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- EP ID EP151574
- DOI 10.31018/jans.v5i1.292
- Views 71
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