Molecular Analysis of Genetic Diversity of Pectobacterium carotovorum Subsp Carotovorum Isolated in Morocco by PCR Amplification of the 16S-23S Intergenic Spacer Region
Journal Title: Biotechnology Journal International - Year 2015, Vol 7, Issue 3
Abstract
Aims: Pectobacterium carotovorum is a ubiquitous bacterium that causes soft rot in different crop plants throughout the world. In Morocco, approximately 95% of the Strains isolates from potato plants with tuber soft rot are P. carotovorum. In this study, we test whether PCR ribotyping can be used to distinguish strains of Pectobacterium carotovorum isolated from soft rot potato and to differentiate among strains from different geographic regions. Place and Duration of Study: Laboratory of Virology, Microbiology and Quality / Ecotoxicology and Biodiversity, department Biology, Faculty of Sciences and Techniques, University Hassan-II Mohammedia Casablanca. Methodology: Eighty-three pectolytic enterobacteria were collected from potatoes rotten in Morocco, the strains were isolated in the Cristal Violet Pectate (CVP) medium and were purified in LPGA agar (yeast extract, peptone, glucose and agar). After purification, strains were identified by physiological and biochemical tests. The confirmation of species was performed by PCR using primers Y1 and Y2. The genetic diversity of Pectobacterium carotovoum was investigated by PCR ribotyping using primers G1/L1, which are complementary to conserved regions of the rRNA operon. Furthermore, the profiles obtained were compared by the Unweighted Pair Group Method. Results: The biochemical and physiological analysis demonstrated that the predominant pectolytic enterobacterium present in Morocco is Pectobacterium carotovorum subsp carotovorum. The specific confirmation of species P. carotovorum by PCR has yielded a 434 bp DNA fragment of the pelY gene with all isolates. Further, PCR amplification of the 16S-23S Intergenic spacer Region (ITS-PCR) has presented a specific pattern made of 2-6 fragments ranging from 300 bp to 800 bp. The UPGMA tree has shown that there is considerable genetic diversity in P. carotovorum strains, which can be divided into four distinct groups.
Authors and Affiliations
H. Faquihi, M. Amdan, R. Ait Mhand
Keywords
Related Articles
- EP ID EP237170
- DOI 10.9734/BBJ/2015/16045
- Views 109
- Downloads 0
Application of Box-Behnken Design for the Optimization of Citric Acid Production from Corn Starch Using Aspergillus niger
Citric acid production from hydrolysed corn starch was optimized in this study. Response surface methodology (RSM) was employed for the analysis of the simultaneous effect of substrate concentration, broth pH and ferment...
Activity of β-Amylase in Some Fungi Strains Isolated from Forest Soil in South-Western Nigeria
Aims: The aim of this study was to isolate fungi species from Omo natural forest soil in Ogun State of Nigeria and study the amylases from the fungi species which are digestive enzymes that hydrolyze glycosidic bonds in...
Optimization of Sweet Potato Starch Hydrolyzate Production and Its Potential Utilization as Substrate for Citric Acid Production
Aims: The aims of this work was optimization of two-step enzymatic hydrolysis of sweet potato starch using statistical approach and subsequent utilization of the hydrolyzate obtained for citric acid production. Methodol...
Ex-situ Conservation of the Rare and Threatened Medicinal Climber Corallocarpus epigaeus Rottler through In vitro Regeneration Method
Aims: To develop in vitro propagation and callus induction protocol for regeneration and conservation of the rare and threatened medicinal climber, Corallocarpus epigaeus, which is widely acclaimed for high medicinal val...
The Effect of Picloram on Somatic Embryogenesis of Different Explants of Strawberry (Fragaria ananassa Duch.)
The present investigation was conducted to study the effects of different concentrations of picloram on somatic embryogenesis induction, development and maturation of three strawberry (Kurdistan, Paros and Camarosa) cult...