Molecular Analysis of Micropropagated Banana Variants (Musa spp. ‘AAA’) Using RAPD Markers
Journal Title: International Journal of Current Microbiology and Applied Sciences (IJCMAS) - Year 2018, Vol 7, Issue 7
Abstract
In this study, RAPD fingerprinting has been carried out for variants selected from orchards growing with micropropagated Robusta banana (Musa spp. ‘AAA’) plants. After isolation of quality DNA and preliminary screening of random primers, fourteen arbitrary (10-mers) primers showing intense unambiguous and reproducible PCR amplification have been selected for study. Among them OPA-19 and OPC-03 showed only monomorphic bands while OPA-02, OPA-09, OPA-13, OPA-14, OPB-6, OPB-15, OPC-01, OPD-10, OPF-04 and OPF-12 primers showed polymorphic bands. A total of 123 amplified fragments were scored of which 53 were polymorphic in nature. In addition, in the dendrogram generated based on Ward’s method of cluster analysis all morphological variants were grouped into a cluster, while other normal plant from orchard along with Robusta and Grand Naine pure lines formed another separate group. The genetic dissimilarity based on Squared Euclidean Distance ranged from 20-35 per cent between morphological variants and normal plant from orchard. The result showed that morphological variations in plants from orchard growing with micropropagated Robusta plants are somaclonal variations, which suggests that the commercial tissue culture units should use molecular means for testing genetic fidelity of tissue culture plants as a safety measure to avoid passing of off-type planting material to farmer. The primers selected in this study are useful in testing somaclonal variants.
Authors and Affiliations
D. Shiddalingeswara, D. P. Prakash, Gajanana Kustagi, V. Nachegowda
Keywords
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- EP ID EP580760
- DOI 10.20546/ijcmas.2018.707.002
- Views 91
- Downloads 0
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