Molecular Analysis of Micropropagated Banana Variants (Musa spp. ‘AAA’) Using RAPD Markers
Journal Title: International Journal of Current Microbiology and Applied Sciences (IJCMAS) - Year 2018, Vol 7, Issue 7
Abstract
In this study, RAPD fingerprinting has been carried out for variants selected from orchards growing with micropropagated Robusta banana (Musa spp. ‘AAA’) plants. After isolation of quality DNA and preliminary screening of random primers, fourteen arbitrary (10-mers) primers showing intense unambiguous and reproducible PCR amplification have been selected for study. Among them OPA-19 and OPC-03 showed only monomorphic bands while OPA-02, OPA-09, OPA-13, OPA-14, OPB-6, OPB-15, OPC-01, OPD-10, OPF-04 and OPF-12 primers showed polymorphic bands. A total of 123 amplified fragments were scored of which 53 were polymorphic in nature. In addition, in the dendrogram generated based on Ward’s method of cluster analysis all morphological variants were grouped into a cluster, while other normal plant from orchard along with Robusta and Grand Naine pure lines formed another separate group. The genetic dissimilarity based on Squared Euclidean Distance ranged from 20-35 per cent between morphological variants and normal plant from orchard. The result showed that morphological variations in plants from orchard growing with micropropagated Robusta plants are somaclonal variations, which suggests that the commercial tissue culture units should use molecular means for testing genetic fidelity of tissue culture plants as a safety measure to avoid passing of off-type planting material to farmer. The primers selected in this study are useful in testing somaclonal variants.
Authors and Affiliations
D. Shiddalingeswara, D. P. Prakash, Gajanana Kustagi, V. Nachegowda
Keywords
Related Articles
- EP ID EP580760
- DOI 10.20546/ijcmas.2018.707.002
- Views 97
- Downloads 0
Effect of Selected Botanicals and Spinosad on Shoot and Fruit Borer (Leucinodes Orbonalis Gueene) and Natural Enemies in Brinjal Ecosystem
A field experiment was conducted during kharif, 2016 at SHUATS, Allahabad (U.P.) to compare the efficacy of selected botanicals, Spinosad against brinjal shoot and fruit borer, (Leucinodes orbonalis Guen.). All the seven...
Effect of Copper Contamination on Soil Biochemical Activity and Performance of Rice (Oryza sativa L.)
A pot culture experiment was carried out to determine the effect of soil contamination with CuSO4 5H2O in different concentrations on the activity of soil enzymes (Dehydrogenases, Urease, Acid and Alkaline Phosphatase) a...
Effect of Jigsaw Co-Operative Learning Method in Improving Cognitive Skills among Medical Students
To shift the paradigm from a passive tutor driven tutorials to more responsible active learning by the students, a cooperative teaching learning method could be a good alternative. The Jigsaw approach, a model of co-oper...
Problems Faced by NGOs Trainees in Adoption of Entrepreneurial Activities
The objective of the present study was to identify the problems faced by NGOs trainees in adoption of entrepreneurial activities. The study was conducted in Gogunda and Kherwara panchayat samities of Udaipur district of...
Isolation and Identification of Extra-aquatic, Pathogenic Fungi from the Polluted Water of Munneru River, Telangana, India
Munneru is a small River and flows through Dornakal Eru, Kaamanchkal and Daanavaayigudem areas of Khammam district, Telangana, India. Munneru is a major water source, the river is so much polluted with the domestic, fact...