Molecular Analysis of Micropropagated Banana Variants (Musa spp. ‘AAA’) Using RAPD Markers
Journal Title: International Journal of Current Microbiology and Applied Sciences (IJCMAS) - Year 2018, Vol 7, Issue 7
Abstract
In this study, RAPD fingerprinting has been carried out for variants selected from orchards growing with micropropagated Robusta banana (Musa spp. ‘AAA’) plants. After isolation of quality DNA and preliminary screening of random primers, fourteen arbitrary (10-mers) primers showing intense unambiguous and reproducible PCR amplification have been selected for study. Among them OPA-19 and OPC-03 showed only monomorphic bands while OPA-02, OPA-09, OPA-13, OPA-14, OPB-6, OPB-15, OPC-01, OPD-10, OPF-04 and OPF-12 primers showed polymorphic bands. A total of 123 amplified fragments were scored of which 53 were polymorphic in nature. In addition, in the dendrogram generated based on Ward’s method of cluster analysis all morphological variants were grouped into a cluster, while other normal plant from orchard along with Robusta and Grand Naine pure lines formed another separate group. The genetic dissimilarity based on Squared Euclidean Distance ranged from 20-35 per cent between morphological variants and normal plant from orchard. The result showed that morphological variations in plants from orchard growing with micropropagated Robusta plants are somaclonal variations, which suggests that the commercial tissue culture units should use molecular means for testing genetic fidelity of tissue culture plants as a safety measure to avoid passing of off-type planting material to farmer. The primers selected in this study are useful in testing somaclonal variants.
Authors and Affiliations
D. Shiddalingeswara, D. P. Prakash, Gajanana Kustagi, V. Nachegowda
Keywords
Related Articles
- EP ID EP580760
- DOI 10.20546/ijcmas.2018.707.002
- Views 93
- Downloads 0
Effect of Poultry Manure and PSB Culture in Conjunction with Different Levels of Phosphorus on Physico-Chemical Properties of Soil of Black Gram (Vigna mungo L.)
The experiment was conducted during kharif (July-October) season 2016 on crop research farm of Department of Soil Science and Agricultural Chemistry, Naini Agricultural Institute, Allahabad. By order to evaluate the effe...
Assessment of Genetic Variability among 78 Cucumber (Cucumis sativus L.) Germplams
An experiment was undertaken to study the variability in 78 cucumber germplasm collected from India as well as from abroad. Wide variations for different horticultural traits were observed under study. Lowest node number...
Variation of Latent Heat Flux and Canopy Temperature over Potato Crop Governed by Soil Moisture
Considering the increased demand of water for agricultural sector estimation of evapo-transpirational (ET) losses becomes significantly important for its proper utilization. The latent energy flux (LE) can provide better...
Performance of Upland Rice Varieties on Different Dates of Sowing in Kymore Plateau Region of Madhya Pradesh, India
An investigation entitled “Effect of sowing-dates on performance of different rice varieties under upland condition” was conducted at the Instructional Farm, College of Agriculture, Rewa (M.P.) under All India Coordinate...
Weather Manifested Rice Cultivation - A Review
Under the scenario of an escalating population and an epoch of climate variability, increasing the production of rice to confront the food security and overcome the human demands from the ever shrinking land resources ha...