Molecular Characterization and Antibiotic Resistance Profile of Bacteria Associated with Clarias gariepinus (Burchell, 1822) From Ebute Iboro Location on Yewa River
Journal Title: Journal of Experimental Agriculture International - Year 2016, Vol 11, Issue 5
Abstract
Bacterial isolates associated with Clarias gariepinus were collected from fish samples gotten on Yewa River in Ebute Iboro. The isolates were assessed using 16S rRNA gene sequencing method to identify the strains and to construct the phylogenetic relationship. The morphometric characteristics such as weight, standard length, total length and head length of fish samples were measured. Bacteria were isolated from the gills, skin and gut of Clarias gariepinius using spread plate method. The DNA of the bacteria isolates were extracted using CTAB method. PCR amplification of 16S ribosomal RNA gene of isolates was carried out using universal primer for bacteria, purification of the PCR product using ethanol precipitation, thereafter sequenced using an automated DNA sequencer. The homologous of nucleotides was then searched at the gene bank of NCBI and the isolates were identified. The antibiotics sensitivity profile of the bacterial isolates was determined using disc diffusion method. Three main types of bacteria. Pseudomonas spp, Serratia spp and Aeromonas spp, were found dominantly on the skin. The sensitivity test showed that the identified bacterial isolates were sensitive to Ofloxacin (5 mg), Nitrofurantoin (300 mg) and Ciprofloxacin (5 mg) which can be used to treat the bacteria isolate to reduce its pathogenic impact on human and to reduce disease outbreak when cultured. The bacterial isolates were 100% resistant to Cefixime (5 mg) and Cefuroxime (30 mg).
Authors and Affiliations
J. K. Ekelemu, A. A. Akinyemi, O. O. Oyelakin, A. R. Oloyede, V. O. Agbasi
Keywords
Related Articles
- EP ID EP351375
- DOI 10.9734/AJEA/2016/19985
- Views 65
- Downloads 0
Fertility Management for Cassava Production in the Centre Region of Cameroon
Cassava is a major food crop in Cameroon. It has the capacity to grow under poor soils or drought conditions. However, little is known about the impact of fertilizer application on cassava production on poor ferralitic s...
Performance of Hand-pollinated Maize Genotypes at Different Daytimes in a Nigeria Forest Agro-ecosystem
Weather condition prevailing during pollination could have some effect on seed set and yield in maize. A controlled pollination experiment was conducted at the Teaching and Research Farm, Ekiti State University, Ado-Ekit...
Determination of Effect of Cold Stratification Temperature and Duration on Germination of Spruce (Picea smithiana Wall. Boiss) under Laboratory Conditions
Aim: The natural regeneration of Picea smithiana is generally slow and almost negligible due to a number of factors e.g. presence of undecomposed raw humus on forest floor, low germinative capacity of seed and long inter...
A Study of Water Dynamics and Phenology in Plant Parts of ‘Eva’ Apple Trees Grown under Winter Conditions in Southern Brazil
Aims: The objective of this study was to evaluate water dynamics in different tissues of apple 'Eva' during dormancy stage under mild winter conditions of Pelotas, located in Southern Brazil. Study Design: Experimental d...
The Influence of NAA, GA3 and Calcium Nitrate on Growth, Yield and Fruit Quality of "Le Conte" Pear Trees
Aims: Investigate the effect of gibberellic acid, naphthalene acetic acid and calcium nitrate on vegetative growth parameters, fruit set, yield, fruit quality and leaf mineral content of "Le Conte" pear trees. Study Desi...