Molecular Characterization and Antibiotic Resistance Profile of Bacteria Associated with Clarias gariepinus (Burchell, 1822) From Ebute Iboro Location on Yewa River
Journal Title: Journal of Experimental Agriculture International - Year 2016, Vol 11, Issue 5
Abstract
Bacterial isolates associated with Clarias gariepinus were collected from fish samples gotten on Yewa River in Ebute Iboro. The isolates were assessed using 16S rRNA gene sequencing method to identify the strains and to construct the phylogenetic relationship. The morphometric characteristics such as weight, standard length, total length and head length of fish samples were measured. Bacteria were isolated from the gills, skin and gut of Clarias gariepinius using spread plate method. The DNA of the bacteria isolates were extracted using CTAB method. PCR amplification of 16S ribosomal RNA gene of isolates was carried out using universal primer for bacteria, purification of the PCR product using ethanol precipitation, thereafter sequenced using an automated DNA sequencer. The homologous of nucleotides was then searched at the gene bank of NCBI and the isolates were identified. The antibiotics sensitivity profile of the bacterial isolates was determined using disc diffusion method. Three main types of bacteria. Pseudomonas spp, Serratia spp and Aeromonas spp, were found dominantly on the skin. The sensitivity test showed that the identified bacterial isolates were sensitive to Ofloxacin (5 mg), Nitrofurantoin (300 mg) and Ciprofloxacin (5 mg) which can be used to treat the bacteria isolate to reduce its pathogenic impact on human and to reduce disease outbreak when cultured. The bacterial isolates were 100% resistant to Cefixime (5 mg) and Cefuroxime (30 mg).
Authors and Affiliations
J. K. Ekelemu, A. A. Akinyemi, O. O. Oyelakin, A. R. Oloyede, V. O. Agbasi
Keywords
Related Articles
- EP ID EP351375
- DOI 10.9734/AJEA/2016/19985
- Views 62
- Downloads 0
Effects of Irrigation Timing on Water Economy, Growth, and Yield of Water Stressed Cotton
Aims: Objectives were to evaluate the effects of timed irrigations on plant biomass and seed-cotton production, plant leaf area, whole-plant transpiration, and transpiration per unit leaf area. Study Design: A complete r...
Study on Consumer Behaviour and Economic Advancements of Gluten-free Products
Gluten-free food was among the top five most-searched words on Google in 2013. During past five years, “gluten” has steadily advanced in consumer online-search interest. Globally gluten free products were estimated to ha...
Production and Quality of Palm Fruits Submitted to Neem Oil
The objective of this study was to evaluate the influence of Neem oil on insect management, on production and quality of palm fruits. The experiment was carried out at the Palma Agricultural Center, Federal University of...
Farmers’ Perceptions of the Effectiveness of the Cocoa Disease and Pest Control Programme (CODAPEC) in Ghana and Its Effects on Poverty Reduction
The study examined the contribution of the Cocoa Disease and Pest Control Programme (CODAPEC), which is a cocoa production-enhancing government policy, to reducing poverty and raising the living standards of cocoa farmer...
Callosobruchus chinensis (L.) (Coleoptera: Bruchidae) Management on Stored Chickpea Using Botanicals in Amhara Region, Ethiopia
Chickpea is an important legume crop in Amhara region. The harvested grain of the crop could be stored for more than six months to look for better price and/or as insurance for food security. In the store Callosobruchus...