Molecular Characterization and Serological Analysis of Dengue Viruses in Amritsar District
Journal Title: International Journal of Contemporary Medical Research - Year 2018, Vol 5, Issue 3
Abstract
Introduction: Dengue is a common mosquito-borne viral disease of humans that in recent years has become a major international public health concern. There are four serotypes of the dengue virus i.e DENV-1, DENV-2, DENV-3, and DENV-4 circulating in Indian population and can cause the full spectrum of disease from a subclinical infection to a mild self-limiting disease, the dengue fever (DF), severe disease that may be fatal, the dengue haemorrhagic fever/dengue shock syndrome (DHF/DSS). In the present study, serological analysis, molecular detection and serotyping of dengue virus was done to look for the prevalent strains of dengue virus in our area. Material and methods: Blood samples were collected from clinically suspected patients of dengue fever selected as per WHO criteria. Samples were screened for the presence of dengue-specific IgM antibodies using MAC-ELISA and dengue NS1 antigen detection was done using Pan Bio (Australia) NS1 ELISA kit, according to the duration of fever as per NVBDCP guidelines. Molecular detection and serotyping was done in 50 NS1 positive samples, by single step multiplex RT-PCR. Results: Total of 3306 samples were tested for dengue. 60.6% were serological positive for were positive for both seromarkers of dengue infection. Seasonal variation of disease was seen and majority of cases tested positive for dengue were obtained in the month of September and October. Male predominance was seen among patients suffering from dengue. Adult age group had higher burden of disease than the pediatric age. Serotypic characterization observed the predominance of DENV-1 (57%) followed by DENV-3 (40%) and DENV-2 (3%) indicating co-circulation of multiple serotypes. Conclusion: Laboratory-based active surveillance systems are needed to complement the current passive surveillance and control programs to detect and monitor sudden increases in the numbers of dengue cases or changes in the predominant serotypes.
Authors and Affiliations
Rubina Paul, Loveena Oberoi, Kanwardeep Singh, Pushpa Devi
Keywords
Related Articles
- EP ID EP430154
- DOI -
- Views 151
- Downloads 0
An Analytical Study of Teenage Deaths in and Around Guntur, Andhra Pradesh, South India
Introduction: The golden days, the great memories, the childhood and teenage, never repeats in human life. Life span of an individual can be differentiated into Childhood, Adolescence, Adulthood and Old age. Teenage is t...
Acute Coronary Syndrome in an Adolescent with Homozygous Familial Hypercholesterolemia
Introduction: Familial hypercholesterolemia is a single gene disorder with autosomal codominant pattern of inheritance. While heterozygous familial hypercholesterolemia is common, homozygous familial hypercholesterolemia...
Estimation of the Prevalence of Tramadol and Cannabis Abuse among Drivers Involved in Road Traffic Accidents Admitted to Alexandria Main University Hospital: A Prospective Study
Introduction: In Egypt, many drivers are regular users of illicit drugs assuming that they reduce tiredness and sleepiness during working hours but unfortunately road traffic accidents result. Cannabis and tramadol are p...
Florid Cemento-Osseous Dysplasia with Multiple Impacted Supernumerary Teeth in Maxilla and Mandible – A Case Report
Introduction: Florid cement-osseous dysplasia is a benign, fibroosseous and multifocal dysplastic lesion of the jaw and consists of cellular fibrous connective tissue with bone and cementum-like tissue. Case report: This...
Management Practices of Juvenile Idiopathic Arthritis among Rheumatologists in India
Introduction: Pediatric rheumatology is a young speciality in India. There is a need to study the awareness and practice of pediatric rheumatological illnesses among practicing rheumatologists and pediatric rheumatologis...