Molecular Characterization and Serological Analysis of Dengue Viruses in Amritsar District
Journal Title: International Journal of Contemporary Medical Research - Year 2018, Vol 5, Issue 3
Abstract
Introduction: Dengue is a common mosquito-borne viral disease of humans that in recent years has become a major international public health concern. There are four serotypes of the dengue virus i.e DENV-1, DENV-2, DENV-3, and DENV-4 circulating in Indian population and can cause the full spectrum of disease from a subclinical infection to a mild self-limiting disease, the dengue fever (DF), severe disease that may be fatal, the dengue haemorrhagic fever/dengue shock syndrome (DHF/DSS). In the present study, serological analysis, molecular detection and serotyping of dengue virus was done to look for the prevalent strains of dengue virus in our area. Material and methods: Blood samples were collected from clinically suspected patients of dengue fever selected as per WHO criteria. Samples were screened for the presence of dengue-specific IgM antibodies using MAC-ELISA and dengue NS1 antigen detection was done using Pan Bio (Australia) NS1 ELISA kit, according to the duration of fever as per NVBDCP guidelines. Molecular detection and serotyping was done in 50 NS1 positive samples, by single step multiplex RT-PCR. Results: Total of 3306 samples were tested for dengue. 60.6% were serological positive for were positive for both seromarkers of dengue infection. Seasonal variation of disease was seen and majority of cases tested positive for dengue were obtained in the month of September and October. Male predominance was seen among patients suffering from dengue. Adult age group had higher burden of disease than the pediatric age. Serotypic characterization observed the predominance of DENV-1 (57%) followed by DENV-3 (40%) and DENV-2 (3%) indicating co-circulation of multiple serotypes. Conclusion: Laboratory-based active surveillance systems are needed to complement the current passive surveillance and control programs to detect and monitor sudden increases in the numbers of dengue cases or changes in the predominant serotypes.
Authors and Affiliations
Rubina Paul, Loveena Oberoi, Kanwardeep Singh, Pushpa Devi
Keywords
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