Molecular characterization of Anaplasma platys in dogs in Pelotas city, Southern Brazil
Journal Title: Scholars Journal of Agriculture and Veterinary Sciences - Year 2016, Vol 3, Issue 1
Abstract
Anaplasma platys belong to the Anaplasmataceae family, infecting platelets of dogs, leading to a disease known as canine cyclic thrombocytopenia (CCT) and are transmitted by ixodid ticks (Rhipicephalus sanguineus). The present study aims to assess the occurrence of this parasite in Pelotas, southern region of Rio Grande do Sul State, Brazil, investigate de clinical and hematological abnormalities and perform its molecular detection. Blood samples from 89 dogs were drawn (from jugular or cephalic veins) in order to carry out red blood count (RBC), platelet count and polymerase chain reaction (PCR/nested PCR) followed by product sequencing. The presence of morulae within platelets was investigated. Results obtained with blood smear showed 1.1% for Anaplasma spp. A total of 7/89 samples (7.9%) were positive for A. platys by nested PCR. In conclusion, the clinical signs and hematological changes detected were nonspecific and common to others diseases. Therefore, it is imperative the use of specific diagnostic techniques in order to identify the etiologic agents. Keywords: canine anaplasmosis, Anaplasmataceae, 16SRNA gene, Anaplasma platys, canine thrombocytopenia, molecular detection, PCR
Authors and Affiliations
Luis Eduardo Barcellos Krause, Luzia Cristina Lencioni Sampaio, Carmen Lucia Garcez Ribeiro, Bianca da Cruz Iéck, Nara Amélia da Rosa Farias, Jerônimo Lopes Ruas
Keywords
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