Molecular Characterization of Cultivated and Wild Genotypes of Punica granatum L. (Pomegranate) by Using SSR Marker
Journal Title: SSR Institute of International Journal of Life Sciences (SSR-IIJLS) - Year 2018, Vol 4, Issue 3
Abstract
The genetic diversity among 20 pomegranate genotypes including cultivated varieties and wild germplasm by using simple sequence repeats (SSR) markers. Plant genomic DNA was isolated using the modified CTAB method. A total of 17 SSR markers were screened across the twenty selected pomegranate germplasm to understand their diversity pattern at a molecular level. Out of these twelve were found to be polymorphic and five were monomorphic. These polymorphic primers have generated 29 SSR marker alleles with an average number of 1.71 alleles per locus. The maximum number of alleles was observed for twelve markers with two alleles each. Polymorphic information content (PIC) values ranged from 0.12 to 0.38 with an average of 0.29 per marker. The observed heterozygosity value ranged from 0.12 to 0.50, with a mean value of 0.36. DARwin software was used to study the phylogenetic relationship among the selected germplasm from the scored data. Neighbor-joining cluster analysis was given three separate clusters. All the wild accessions were grouped into cluster I, while cultivated varieties in cluster II. Single accession (Ruby) was formed a unique cluster. PgSSR33, PgSSR16, and PgSSR25 marker were found highly polymorphic, can be efficiently used in future pomegranate breeding programs.
Authors and Affiliations
Sagar R Mahajan, Vaishali Mahajan, Bhosale SS
Keywords
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- EP ID EP670909
- DOI 10.21276/ijlssr.2018.4.3.8
- Views 102
- Downloads 0
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