Molecular Characterization of Lettuce mosaic virus Isolates in Ankara Province
Journal Title: International Journal of Agriculture Innovations and Research - Year 2018, Vol 6, Issue 5
Abstract
Lettuce (Lactuca sativa) is an important vegetable crop with increasing economic value in Ankara province of Turkey. Many viruses cause diseases and economic losses in lettuce production and among them Lettuce mosaic virus (LMV), is one of the most common virus infection. In this present research, 324 symptomatic and asymptomatic lettuce samples were collected from five districts of Ankara province (Beypazarı, Çubuk, Ayaş, Nallıhan and Centrum) in May-July, 2015 and all of the collected samples were subjected to serological test of double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) and among them, 25 samples were identified as solely infected with LMV. All of the selected isolates were confirmed by reverse transcription-polymerase chain reaction (RT-PCR) assay. Coat protein gene specific primer pairs were used and amplified products were 800 bp long. Amplified cDNA fragments of Beypazari, Cubuk, Ayas isolates were sequenced for molecular characterization and the detection of genetic diversity of LMV Ankara isolates. Sequence comparisons showed 89-99%, 93-100 % and 57-100% sequence identity at nucleotide, amino acid level and N-terminus regions, respectively, of the CP gene of LMV isolates. The CP gene of the LMV Ankara isolates were clustered with Western European, Latin American, North African isolates.
Authors and Affiliations
Filiz Randa Zelyut, et al.
Keywords
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