MOLECULAR CHARACTERIZATION OF MECA GENE IN METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS
Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2017, Vol 4, Issue 2
Abstract
The present study aimed to detect the molecular profile of methicillin-resistant Staphylococcus aureus (MRSA) isolated from skin abscess patients in Nassyriah City, Southern Iraq. During the period of from June 2014 to February 2015, a 120 S. aureus isolates were enrolled, and only 64 diagnosed phenotypically as MRSA (53.33%). Conventional polymerase Chain Reaction technique was used for the amplification of 310 bp mecA gene. The results showed that 88/120 (73.33%) of S. aureus were positive for the targeted gene. Three PCR products of mecA S. aureus isolates named primarily as (THQR1, THQR2, and THQR3), were selected and subjected to partial DNA sequencing for the mecA gene to follow up their possible molecular relationship between these local isolates and what recorded globally in Genbank. The Three PCR products of mecA S. aureus isolates were granted Genbank accession numbers of (KY468502, KY468503 and KY468504, respectively). The constructed phylogenetic tree showed that S. aureus KY468502 and KY468504 were highly relative to each other in comparison with S .aureus KY468503 that revealed a close relatedness to S. aureus TN/CN/1/12, mecA gene beta-lactam, and mecA gene isolated in USA. The present study results highlighted the increasing manner of MRSA prevalence in Iraq and the importance of molecular techniques as an epidemiology indicator.
Authors and Affiliations
Saad S. Hamim
Keywords
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