Molecular Characterization of Mycobacterium tuberculosis complex in Clinical Isolates
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2017, Vol 5, Issue 8
Abstract
Abstract:Tuberculosis (TB) is an infectious bacterial disease caused by Mycobacterium tuberculosis, typically affects the lungs, but it can also affect other parts of the body. Diagnostics methods includes Serological, Radiological and Microbiological investigations. The current study was done for doing comparison between the specificity and sensitivity of ZN staining , culture and conventional PCR. 50 clinical isolates were collected and subjected for AFB smear preparation, Culture and Conventional -PCR (IS6110) for mycobacterium tuberculosis complex detection. 5 came positive for AFB smears, out of which 02 were pulmonary and 03 were extra pulmonary. 3 came positive for culture, out of which 01 was pulmonary and 02 were extra pulmonary. Conventional- PCR targeting IS6110 gene was amplified at 123 base pairs with 340 base pairs as IC (internal control) was seen in 9 cases which include 2 pulmonary and 7 extra pulmonary. Thus, PCR was highly reliable for detection and conformation of Mycobacterium tuberculosis bacilli than AFB stain and Culture. This study showed that PCR can be considered as an alternative to ZN in combination with culture for the diagnosis of TB. Hence PCR is rapid and sensitive method for the early diagnosis of pulmonary and extra-pulmonary tuberculosis. Keywords:Mycobacterium Tuberculosis complex, Conventional-PCR, Amplicons, Lowenstein-Jensen medium, Endometrial biopsy, Cerebrospinal Fluid
Authors and Affiliations
Sourav Garg, , Narotam Sharma
Keywords
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