Molecular Characterization of Putative Calcitonin Gene-Related Peptide Receptors and Expression of Calcitonin Gene-Related Peptide and its Receptor in the Early Development of Flounder, Paralichthys olivaceus
Journal Title: International Journal of Zoological Investigations - Year 2018, Vol 4, Issue 2
Abstract
Calcitonin gene-related peptide (CGRP) is a 37-amino acid neuropeptide that has been identified in teleosts and mammals. Calcitonin receptor-like receptor (CLR) forms the CGRP receptor. In teleost fish, CGRP plays a role in vasodilation and is associated with osmotic regulation. However, the embryonic function of CGRP remains unclear. Therefore, we have investigated CGRP function related to early development in the flounder, Paralichthys olivaceus. We first identified two CLRs from the flounder genome database. According to molecular phylogenetic analysis, two CLRs are classified in the group formed by teleost CLR1 and 2. They represented high amino acid sequence identity among mefugu CLR1-3 and human CLR. We also identified another CLR/CTR candidate from the flounder genome. Molecular phylogenetic analysis revealed that this gene was located at the clade formed by vertebrate CTRs that are CLR paralogs. The deduced protein of the gene also had four hormone-binding domains, which are the typical feature of teleost CTR, and was completely different from the CLR of teleosts, including flounder CLRs. These data strongly suggest that flounder possess two putative CGRP receptors. Finally, we performed the expression analysis of flounder CGRP and two CLRs in the flounder embryo. Both expressions of CGRP mRNA and two CLR mRNAs were detected from 96 hours post fertilization (hpf). These results demonstrate that CGRP has some functions at 96 hpf. At that stage, embryos have already hatched and the circulatory system functions, whereas digestive organs still continue to develop. Taken together, these findings suggest that the CGRP peptide may play a role in the circulatory system, as in adult fish, or it may be associated with the development of the digestive tract.
Authors and Affiliations
Nobuo Suzuki
Keywords
Related Articles
- EP ID EP451482
- DOI -
- Views 138
- Downloads 0
Paenibacillus odorifer Berge et al., a New Bacterial Record From India as a Fish Pathogen
A new bacterial form, Paenibacillus odorifer, has been recorded as primary invader on Labeo rohita fish and identified by genomic DNA and 16 S r-RNA sequence analysis. Paenibacillus odorifer has been reported, for the fi...
Impact of Global Climate Warming on Animal Ecology and Human Health
During the last fifty years, a rapid increase in temperature has been reported on the earth’s surface as well as in the oceans. Climate change is reflected in extreme weather conditions such as tsunamis, cyclones, heat w...
Insecticidal Action of Pyrethrum Extract on the Carbohydrate and Phosphatase Biochemistry of the Larva of Rice-Moth, Corcyra cephalonica Staint. (Lepidoptera : Pyralidae)
Sub-lethal doses ( 0.0001, 0.0002 and 0.0004% ) of pyrethrum extract caused a significantly dosedependent reduction in glycogen level and alkaline phosphatase activity and a significantly dose dependent enhancement in re...
Drosophila as a Powerful Modern Tool for Teaching Concepts in Biology and Biomedical Research
Drosophila is a potential teaching tool to help students in understanding core concepts in biology. Flies have gained significant importance as one of the forefront model organism for research in different areas of Biolo...
Sequencing and Expression Analysis of Calcitonin Receptor in the Scales of Goldfish, Carassius auratus
In this study, we have determined the partial nucleotide sequence of calcitonin receptor (CTR) from the scales of goldfish (Carassius auratus). The obtained goldfish CTR cDNA coded a 502-amino-acid protein. The amino aci...