Molecular Characterization of Vancomycin Resistance Enterococcusfaeciumclinical Isolates and Efficacy of Ozone on Pattern of Antibiotic(s) Resistance Bacterial Cell wall Using SEM.
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 16, Issue 2
Abstract
Sixty isolates ofVancomycin Resistant Enterococcus faecium (VRE) were collected from King Faisal Specialist Hospital in Riyadh, Saudi Arabia.These were collected from all clinical sample including surveillance cultures. Out of the 60 isolates, 21 isolates were subjected to multiplex polymerase chain reaction (MPCR) technique.Ten out of 21isolates (47.6%) carried a single vanA gene, while 6 isolates (28.6%) had singlevanB gene. Rest of the isolates carried vanC (23.8%) with either vanA (14.3%) or vanB(9.5%). The efficiency of ozone in converted of VRE isolated bacteria sensitivity from resistant to sensitive towards antibiotic(s) was recorded.Moreover, the effect of different flow rates of ozone (2, 4, 6L/min) on the cell wall of tested bacteria were observed by using Scanning Electron Microscope (SEM). The bacterial cells showed change, and some deformities in the external cell wall. In addition, some of bacterial cells showed early stage of cell lysis, as displayed lacking its integrity by forming bubble – like shape. Treatment with ozone led to decrease number of cells, comparison with non-treated cells.
Authors and Affiliations
Madiha A. Al-Enazi, Alia A. Shoeib
Keywords
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- EP ID EP592014
- DOI 10.9790/0853-1602043845.
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