Molecular detection and characterization of Canine distemper virus from domestic dogs (Canis familiaris) in Quezon City, Philippines
Journal Title: International Journal of Life Science - Year 2017, Vol 5, Issue 4
Abstract
Canine distemper (CD) is a highly infectious viral disease affecting all terrestrial carnivores worldwide. In spite of the availability of commercial modified live vaccines, several studies have reported the occurrence of CD in vaccinated animals. In this study, six dogs with clinical signs of CD were investigated. The age of the dogs ranged from 2 months to 8 years old, two of which were unvaccinated, while the others have outdated vaccination. General clinical signs observed were twitching, myoclonus, diarrhea, oculonasal discharge, and lethargy. Laboratory results revealed presence of anemia and lymphopenia. Oculonasal swabs were collected and nested reverse transcription polymerase chain reaction (nRT-PCR) was performed. Nucleotide sequencing of the hypervariable region of the hemagglutinin (H) gene showed that the field CDV strains belong to the Asia-4 lineage, a new CDV lineage first reported in Thailand in 2013. Nucleotide sequence similarity showed that the field CDVs were closely related (94%) to CDV strains from infected dogs in Thailand. Possible genetic evolution due to interspecies transmission and animal movement such as importation or international transportation of dogs and other susceptible carnivores are suspected. At present, this is the first study to molecularly characterize CDVs in the Philippines.
Authors and Affiliations
Czarina Kristine D Nobleza, Maria Rosario S Racho, Dennis V Umali, Hiromitsu Katoh
Keywords
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