Molecular detection of Anaplasma marginale and Anaplasma ovis in sheep and goat in west highland pasture of Iran
Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2017, Vol 7, Issue 5
Abstract
Objective: To determine the prevalence of Anaplasma marginale (A. marginale) and Anaplasma ovis from sheep and goat in different highland pasture in west of Iran. Methods: From July 2015 to October 2015, 370 blood samples of sheep and goat were collected from different regions in Hamedan province, Iran. The DNA extracted from blood and subsequently, 16S rRNA and MSP4 genes were analyzed by nested-PCR, semi nested-PCR and RFLP methods. Results: In the PCR assessment, overall 27.5% (102/370) of sheep and goat were positive for Anaplasma ovis and A. marginale infection, which was lower than reports from tropical and subtropical regions of Iran. Statistical analysis (the Chi-square test) did not show any significant relation between infection and variables such as location, tick infestation age and sex (P > 0.05). No significant correlation between the altitude and the Anaplasma species infection was found (Mann–Whitney test: P > 0.05). However, Anaplasma infection in goat significantly is more than the sheep (P = 0.008). Conclusions: The ecological changes affect the frequency and distribution of Anaplasma species. Furthermore, our results indicate that sheep as potential reservoirs of A. marginale.
Authors and Affiliations
Ali Yousefi, Sadegh Rahbari, Zainab Sadeghi-dehkordi, Alireza Bahonar
Keywords
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- EP ID EP253662
- DOI 10.1016/j.apjtb.2017.01.017
- Views 63
- Downloads 0
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