Molecular detection of Burkholderia pseudomallei in patients with suspected pulmonary and extra pulmonary tuberculosis
Journal Title: Journal of Microbiology and Infectious Diseases - Year 2017, Vol 7, Issue 1
Abstract
Objectives: Since melioidosis mimics tuberculosis clinically and radiologically, there is a need for a rapid diagnostic method to help the clinician to initiate appropriate antimicrobial treatment in order to prevent mortality. Our objective was to standardize a nested PCR for B. pseudomallei and its detection in pulmonary and extra pulmonary samples from patients with suspected TB. Materials and Methods: Archived pulmonary and extra pulmonary samples which were negative for M. tuberculosis smear microscopy, culture and PCR were included in the study. DNA was extracted (QiAmp Blood DNA kit, Qiagen, Germany) and conventional nested PCR were carried out to detect the presence of 16S-23S spacer region of B. pseudomallei. The DNA was detected by 2% agarose gel electrophoresis and the presence of 251 bp was considered positive. Results: A total of 55 samples were tested, out of which 9 (16.3%) samples tested positive for Burkholderia pseudomallei using nested PCR, which included 5 extra pulmonary and 4 pulmonary samples. These patients belonged to Tamil Nadu 8 (88.8%) and West Bengal 1 (11.1%) both of which are rice growing regions. Among the nine patients who were positive for B. pseudomallei by nested PCR, 2 (22%) were receiving empirical anti-tubercular treatment (ATT). Also, these patients encountered co-morbid condition like renal failure, malignancy, diabetes and co-infection with HIV. Conclusion: We suggest that the patients with symptoms suggestive of both pulmonary and extra pulmonary tuberculosis should be routinely tested for Burkholderia pseudomallei by molecular methods for timely initiation of appropriate therapy and avoid unnecessary exposure to ATT. J Microbiol Infect Dis 2017; 7(1): 21-28
Authors and Affiliations
Evangeline Jayakumar, Ramya Barani, Vigna Seshan, Monika Mani, Sudhakar Muthiah Kothandaramanujam, Rajagopalan Balakrishnan, Padma Srikanth
Keywords
Related Articles
- EP ID EP413351
- DOI 10.5799/ jmid.vi.328840
- Views 110
- Downloads 0
Staphylococcus lugdunensis bacteraemia secondary to an infected intracardiac fragment of a vascular access device
We report a case of Staphylococcus lugdunensis bacteraemia following infection of a fragmented totally implantable vascular access device. This report highlights the virulence of S. lugdunensis especially in immunosuppre...
Cascade of Care for HIV Positive Patients in a Rural Health Region in the North-Central Region of Ivory Coast: A Follow-up Study
Objective: To describe the cascade of care for HIV positive patients in the health region of Worodougou-Béré, North-Central Region of Ivory Coast. Methods: Cross-sectional study which included patients diagnosed HIV posi...
Hookworm in the terminal ileum:a common cause of severe anemia residing in a rare location
Adult Hookworms usually live in the duodenum and jejunum and can be recovered endoscopically for the diagnosis of chronic anemia. This report describes an interesting case where adult hookworm (Ancylostoma duodenale) was...
Increasing penicillin resistance in pneumococci isolated from cerebrospinal fluid samples: Fifteen-year experience from a teaching hospital
Objective: There have been prominent changes in evaluation of resistance patterns of pneumococci and breakpoint values in recent years. We aimed to investigate the penicillin sensitivity of pneumococcal strains isolated...
Development of Simplex-PCR Assays for Accurate Identification of Nine Staphylococcal Species at Genus and Species Levels
Development of Simplex-PCR Assays for Accurate Identification of Nine Staphylococcal Species at Genus and Species Levels Bibek Ranjan Shome1, Krithiga Natesan1, Susweta Das Mitra1,2, Nimita Venugopal1, Bhuvana Mani1, Fer...