Molecular Detection of Hepatitis B Viruses (HBV)
Journal Title: International Blood Research & Reviews - Year 2017, Vol 7, Issue 1
Abstract
Aims: HBV causes both acute and chronic infections and is transmitted through blood or other body fluids. The present study deals with the molecular detection of HBV using PCR. Study Design: Confirmed positive samples of HBV were used to standardize the molecular diagnostic assay. DNA was extracted and used to standardize diagnostic PCR. Place and Duration of Study: Centre for Biotechnology, Maharshi Dayanand University, Rohtak, Haryana, India, between January 2015 and July 2015. Methodology: Positive Samples were obtained from Department of Medicine, Maulana Azad Medical College (MAMC), New Delhi. DNA was extracted from these positive samples and used for standardization of conventional PCR reaction. The results were checked by gel electrophoresis. Results: Positive samples of HBV were detected by standardize PCR. Both the samples showed strong band of 259 bp and there is no amplification in the negative control. Conclusion: Rapid tests have low sensitivity and specificity while molecular assays are rapid, sensitive and specific. Conventional PCR is rapid, specific, sensitive and it is also less costly than Real-Time PCR. Cost of an assay is an important factor in controlling a disease in developing countries.
Authors and Affiliations
Vikrant Sharma, Deepak Kumar, Divya Dhull, Sulochana Kaushik, Samander Kaushik
Keywords
Related Articles
- EP ID EP306678
- DOI 10.9734/IBRR/2017/31171
- Views 125
- Downloads 0
A Preliminary Study of the Effect of Alchornea laxiflora on Haematological Parameters and Organ Weight in Albino Rats
Aim: The aim of this study is to investigate the effects of ethanol extract of Alchornea laxiflora on internal organs as well as haematological indices of rats. Methodology: The plant materials were air dried and grinded...
Iron Overload, Chelation Therapy and Survival in Lower-Risk Myelodysplastic Syndromes: State of the Evidence
Myelodysplastic syndromes (MDS) are clonal stem cell disorders that primarily affect older persons and are associated with peripheral blood cytopenias, increased risk of conversion to acute myeloid leukemia and shortened...
Knowledge, Attitude and Practice of Blood Conservation Strategies amongst Physicians’ in Tertiary Hospitals
Aim: To assess the extent of available knowledge, as well as the attitudes and the utilization of blood conservation strategies amongst medical doctors, who are the implementers of blood transfusion and conservation in c...
Knowledge and Beliefs of Blood Cancer among Affected and Unaffected Persons in Benin City, Nigeria
Introduction: Awareness and adequate knowledge regarding blood cancers is a crucial intervention for proper management and control. Objectives: This study aimed to quantify and compare the level of knowledge among affec...
Prevalence of HIV Infection among Blood Donors at a Tertiary Care Centre in Gwalior, India
Background: Transfusion Transmitted Infections (TTIs) threaten safety of the recipients and the community as a whole and are the subject of real concern worldwide. Human immunodeficiency virus (HIV) causes Acquired Immun...