Molecular detection of mouse hepatitis virus in laboratory mouse colonies
Journal Title: Iranian Journal of Veterinary Science and Technology (IJVST) - Year 2019, Vol 11, Issue 2
Abstract
The animal health monitoring is required to issue health certificates. The viral hepatitis virus is one of the most important infectious agents in mice breeding colonies. This research used RT-PCR to identify contaminations to mouse hepatitis virus. 18 out of 29 specimens were found to be infected, a prevalence of 62%. PCR product was purified and sequenced. Phylogenetic analysis revealed that the identified strain in this study was closely related to a strain reported from France. In the conventional system, contamination with different infectious agents is inevitable, thus it is better to replace the contaminated colonies with clean animals.
Authors and Affiliations
Roozbeh Fallahi,Fatemeh Abedini,Gholam Reza Shokri,
Prevalence ofMelophagus ovinus (Diptera, Hippoboscidae) in sheep in the province of Tungurahua, Ecuador
Melophagus ovinus (Diptera: Hippoboscidae) is considered one of the main ectoparasites that attack sheep, however, in Ecuador information about this parasite is limited. In this study, the prevalence of Melophagus ovinus...
Molecular Identification of Mycobacterium avium subsp. Paratuberculosis isolated from ELISA-Positive Samples by Nested PCR
Paratuberculosis (Johne's disease) is a chronic granulomatous small intestine disease caused by MAP. Diagnosing and isolating infected animals is the most important measure for controlling the disease. Therefore, this st...
Histopathologic report of infestation by Centrocestus formosanus in Iranian grass carp and common carp
Flukes cause severe and lethal diseases in various animals comprising fish. Both adult and larval stages are found in fish. Centrocestiasis is an infection of the fish gills by heterophyid trematode Centrocestus formosan...
Sensory evaluation of the color of mutton by computer vision system
Evaluation of meat color by a computer vision system (CVS) is a promising implement to dominate the difficulties when the meat is directly evaluated. In this study, 60 Longissimus dorsi from different carcasses of sheep...
Evaluation of supracondylar femur fractures in cats: a retrospective study
Femoral fractures in cats are common and are usually treated using pins and wires, lag screws, intramedullary pins, as well as interlocking pins and plates. Among femur fractures, supracondylar femur fractures have an im...